MediaDive DSMZ
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder Unit converter Compare media Compare solutions Prediction SOON
DSMZ

DSMZ 104 . PYG MEDIUM (modified)

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×
Medium for Abiotrophia defectiva DSM 9849

This strain uses the standard medium without modifications.

Growth conditions: 37 °C

Main sol. 104 #

ml
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Trypticase peptone 5.0 g 5 -
Peptone 5.0 g 5 -
Yeast extract 10.0 g 10 -
Beef extract 5.0 g 5 -
Glucose 5.0 g 5 27.753
K2HPO4 2.0 g 2 11.483
Tween 80 1.0 ml - -
Salt solution 40.0 ml - -
Resazurin 1.0 mg 1.0e-3 0.004
Vitamin K1 solution 0.2 ml - -
Haemin solution 10.0 ml - -
L-Cysteine HCl x H2O 0.5 g 0.5 -
Distilled water 950.0 ml - -
1 The vitamin K1, haemin solution and the cysteine are added after the medium has been boiled and cooled under CO2. Adjust pH to 7.2 using 8 N NaOH. Distribute under N2 and autoclave.

Salt solution #

1000
Solution occurs in 9 other media (first defined in Medium 104).
Compound Amount Unit Conc. [g/L] Conc. [mM]
CaCl2 x 2 H2O 0.25 g 0.25 1.701
MgSO4 x 7 H2O 0.50 g 0.5 2.029
K2HPO4 1.00 g 1 5.741
KH2PO4 1.00 g 1 7.348
NaHCO3 10.00 g 10 119.038
NaCl 2.00 g 2 34.223
Distilled water 1000.00 ml - -

Haemin solution #

100
Solution occurs in 15 other media (first defined in Medium 78).
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Haemin 50 mg 0.5 0.767
NaOH
(1 N) 		1 ml - -
Distilled water 100 ml - -
1 Dissolve 50 mg haemin in 1 ml 1 N NaOH; make up to 100 ml with distilled water and filter sterilize. Store refrigerated.

Vitamin K1 solution #

20
Solution occurs in 12 other media (first defined in Medium 78).
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin K1 0.1 ml - -
Ethanol
(95 %) 		20.0 ml 950 20620.8
1 Dissolve 0.1 ml of vitamin K1 in 20 ml 95% ethanol and filter sterilize. Store refrigerated in a brown bottle.

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.2
Equipment needed: Autoclave, Filter
Edit in Medium builder

Associated strains

All strains for this medium: 483
Strains with modifications: 103
Abiotrophia defectiva DSM 9849

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Yeast extract 10 -
Glucose 5 27.753
Peptone 5 -
Trypticase peptone 5 -
Beef extract 5 -
Show more
CSV
JSON

Cultivation metadata from 483 strains BacDive

halophily non-halophilic 1 strain
pH 6 - 8 33 strains
pH 4 - 6 14 strains
pH 8 - 10 13 strains
phyla 13 465 strains
Show more