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DSMZ

DSMZ 104 . PYG MEDIUM (modified)

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×
Medium for Phocaeicola coprophilus DSM 18228

This strain uses the standard medium without modifications.

Growth conditions: 37 °C

Main sol. 104 #

ml
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Trypticase peptone 5.0 g 5 -
Peptone 5.0 g 5 -
Yeast extract 10.0 g 10 -
Beef extract 5.0 g 5 -
Glucose 5.0 g 5 27.753
K2HPO4 2.0 g 2 11.483
Tween 80 1.0 ml - -
Salt solution 40.0 ml - -
Resazurin 1.0 mg 1.0e-3 0.004
Vitamin K1 solution 0.2 ml - -
Haemin solution 10.0 ml - -
L-Cysteine HCl x H2O 0.5 g 0.5 -
Distilled water 950.0 ml - -
1 The vitamin K1, haemin solution and the cysteine are added after the medium has been boiled and cooled under CO2. Adjust pH to 7.2 using 8 N NaOH. Distribute under N2 and autoclave.

Salt solution #

1000
Solution occurs in 9 other media (first defined in Medium 104).
Compound Amount Unit Conc. [g/L] Conc. [mM]
CaCl2 x 2 H2O 0.25 g 0.25 1.701
MgSO4 x 7 H2O 0.50 g 0.5 2.029
K2HPO4 1.00 g 1 5.741
KH2PO4 1.00 g 1 7.348
NaHCO3 10.00 g 10 119.038
NaCl 2.00 g 2 34.223
Distilled water 1000.00 ml - -

Haemin solution #

100
Solution occurs in 15 other media (first defined in Medium 78).
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Haemin 50 mg 0.5 0.767
NaOH
(1 N) 		1 ml - -
Distilled water 100 ml - -
1 Dissolve 50 mg haemin in 1 ml 1 N NaOH; make up to 100 ml with distilled water and filter sterilize. Store refrigerated.

Vitamin K1 solution #

20
Solution occurs in 12 other media (first defined in Medium 78).
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin K1 0.1 ml - -
Ethanol
(95 %) 		20.0 ml 950 20620.8
1 Dissolve 0.1 ml of vitamin K1 in 20 ml 95% ethanol and filter sterilize. Store refrigerated in a brown bottle.

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.2
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 483
Strains with modifications: 103
Phocaeicola coprophilus DSM 18228

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Yeast extract 10 -
Glucose 5 27.753
Peptone 5 -
Trypticase peptone 5 -
Beef extract 5 -
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Cultivation metadata from 483 strains BacDive

halophily non-halophilic 1 strain
pH 6 - 8 33 strains
pH 4 - 6 14 strains
pH 8 - 10 13 strains
phyla 13 465 strains
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