1002
1002 .
ECTOTHIORHODOSYNUS MEDIUM
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Main sol. 1002 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 0.5 | g | 0.5 | 3.674 | |
| NH4Cl | 0.5 | g | 0.5 | 9.348 | |
| NaCl | 20.0 | g | 20 | 342.231 | |
| MgCl2 x 6 H2O | 0.2 | g | 0.2 | 0.984 | |
| CaCl2 | 0.1 | g | 0.1 | 0.901 | |
| Na2CO3 | 5.0 | g | 5 | 47.175 | |
| NaHCO3 | 5.0 | g | 5 | 59.519 | |
| Sodium acetate | 1.0 | g | 1 | 12.191 | |
| Yeast extract | 0.1 | g | 0.1 | - | |
| Na2S2O3 x 5 H2O | 0.5 | g | 0.5 | 2.015 | |
| Na2S x 9 H2O | 0.1 | g | 0.1 | 0.416 | |
| Trace element solution SL-6 | 1.0 | ml | - | - | |
| Vitamin B12 | 20.0 | µg | 2.0e-5 | 1.5e-5 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Prepare the NH4Cl, CaCl2, carbonate, bicarbonate and sulfide as a single sterile stock solution and add to the sterile medium after autoclaving. The pH should be adjust to 9-9.5. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| ZnSO4 x 7 H2O | 0.10 | g | 0.1 | 0.348 |
| MnCl2 x 4 H2O | 0.03 | g | 0.03 | 0.152 |
| H3BO3 | 0.30 | g | 0.3 | 4.851 |
| CoCl2 x 6 H2O | 0.20 | g | 0.2 | 1.54 |
| CuCl2 x 2 H2O | 0.01 | g | 0.01 | 0.059 |
| NiCl2 x 6 H2O | 0.02 | g | 0.02 | 0.154 |
| Na2MoO4 x 2 H2O | 0.03 | g | 0.03 | 0.124 |
| Distilled water | 1000.00 | ml | - | - |
Metadata
| Last modified: | 25.03.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 9.0 - 9.5 |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 1 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaCl | 20 | 342.231 |
| NaHCO3 | 5 | 59.519 |
| Na2CO3 | 5 | 47.175 |
| Sodium acetate | 1 | 12.191 |
| NH4Cl | 0.5 | 9.348 |
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