Main sol. 1002
Internal Solution-ID: S2045
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 0.5 | g | 0.5 | 3.674 | |
| NH4Cl | 0.5 | g | 0.5 | 9.348 | |
| NaCl | 20.0 | g | 20 | 342.231 | |
| MgCl2 x 6 H2O | 0.2 | g | 0.2 | 0.984 | |
| CaCl2 | 0.1 | g | 0.1 | 0.901 | |
| Na2CO3 | 5.0 | g | 5 | 47.175 | |
| NaHCO3 | 5.0 | g | 5 | 59.519 | |
| Sodium acetate | 1.0 | g | 1 | 12.191 | |
| Yeast extract | 0.1 | g | 0.1 | - | |
| Na2S2O3 x 5 H2O | 0.5 | g | 0.5 | 2.015 | |
| Na2S x 9 H2O | 0.1 | g | 0.1 | 0.416 | |
| Trace element solution SL-6 | 1.0 | ml | - | - | |
| Vitamin B12 | 20.0 | µg | 2.0e-5 | 1.5e-5 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Prepare the NH4Cl, CaCl2, carbonate, bicarbonate and sulfide as a single sterile stock solution and add to the sterile medium after autoclaving. The pH should be adjust to 9-9.5. | |||||
Media with this solution
| 1002 | ECTOTHIORHODOSYNUS MEDIUM |