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The medium composition is currently in its beta status, meaning that some concentrations are not correct and need further adjustments. Keep this in mind while trying this out.



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Current criteria: NaHCO3

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28 PFENNIG'S MEDIUM I

Compound Conc. [g/L] Conc. [mM]
NaHCO3 1.5 17.856
Na2S x 9 H2O 0.593 2.467
MgSO4 x 7 H2O 0.5 2.029
Ammonium chloride 0.35 6.543
KCl 0.35 4.695
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Solutions:
  • 460 ml Solution A
  • 40 ml Sulfide solution, 1.5%
  • 50 ml Bicarbonate solution
  • 450 ml Resazurin solution
  • 26 ml Pfennig's heterotrophic salts solution
  • 1 ml Vitamin B12 solution
  • 10 ml Neutralized sulfide solution 3% (w/v)
  • 1 ml Trace element solution SL-12 B

28a THIORHODOCOCCUS MEDIUM

Compound Conc. [g/L] Conc. [mM]
NaCl 20 342.231
NaHCO3 1.5 17.856
MgCl2 x 6 H2O 1 4.919
Na2S x 9 H2O 0.593 2.467
MgSO4 x 7 H2O 0.5 2.029
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Solutions:
  • 460 ml Solution A
  • 40 ml Sulfide solution, 1.5%
  • 50 ml Bicarbonate solution
  • 450 ml Resazurin solution
  • 26 ml Pfennig's heterotrophic salts solution
  • 1 ml Vitamin B12 solution
  • 10 ml Neutralized sulfide solution 3% (w/v)

28b THIORHODOCOCCUS MEDIUM II

Compound Conc. [g/L] Conc. [mM]
NaCl 15 256.674
MgCl2 x 6 H2O 2.5 12.297
NaHCO3 1.5 17.856
Na2S x 9 H2O 0.593 2.467
MgSO4 x 7 H2O 0.5 2.029
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Solutions:
  • 460 ml Solution A
  • 40 ml Sulfide solution, 1.5%
  • 50 ml Bicarbonate solution
  • 450 ml Resazurin solution
  • 26 ml Pfennig's heterotrophic salts solution
  • 1 ml Vitamin B12 solution
  • 10 ml Neutralized sulfide solution 3% (w/v)

29 PFENNIG'S MEDIUM II

Compound Conc. [g/L] Conc. [mM]
NaHCO3 1.5 17.856
Na2S x 9 H2O 0.593 2.467
MgSO4 x 7 H2O 0.52 2.11
Ammonium chloride 0.364 6.805
KCl 0.364 4.883
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Solutions:
  • 460 ml Solution A
  • 40 ml Solution B
  • 50 ml Solution C
  • 450 ml Solution D
  • 26 ml Solution E
  • 10 ml Solution F
  • 1 ml Trace element solution SL-10 B

29a MEDIUM FOR CHLOROBIUM FERROOXIDANS

Compound Conc. [g/L] Conc. [mM]
FeSO4 1.519 10
NaHCO3 1.5 17.856
MgSO4 x 7 H2O 0.52 2.11
Ammonium chloride 0.364 6.805
KCl 0.364 4.883
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Solutions:
  • 1 ml Trace element solution SL-10 B
  • 460 ml Solution A
  • 40 ml Solution B
  • 50 ml Solution C
  • 450 ml Solution D
  • 26 ml Solution E
  • 10 ml Solution F

31 ALKALINE NUTRIENT AGAR

Compound Conc. [g/L] Conc. [mM]
Agar 15 44.599
Na2CO3 anhydrous 5.3 50.006
Peptone 5 -
NaHCO3 4.2 49.996
Meat extract 3 -
Distilled water - -
Solutions:
  • 100 ml Na-sesquicarbonate solution

39 PFENNIG'S MEDIUM I WITH YEAST EXTRACT

Compound Conc. [g/L] Conc. [mM]
NaHCO3 1.5 17.856
Na2S x 9 H2O 0.593 2.467
MgSO4 x 7 H2O 0.5 2.029
Yeast extract 0.5 -
Ammonium chloride 0.35 6.543
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Solutions:
  • 460 ml Solution A
  • 40 ml Sulfide solution, 1.5%
  • 50 ml Bicarbonate solution
  • 450 ml Resazurin solution
  • 26 ml Pfennig's heterotrophic salts solution
  • 1 ml Vitamin B12 solution
  • 10 ml Neutralized sulfide solution 3% (w/v)
  • 1 ml Trace element solution SL-12 B

40 PFENNIG'S MEDIUM II WITH SALT

Compound Conc. [g/L] Conc. [mM]
NaCl 10 171.116
NaHCO3 1.5 17.856
MgSO4 x 7 H2O 0.52 2.11
Ammonium chloride 0.364 6.805
KCl 0.364 4.883
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Solutions:
  • 460 ml Solution A
  • 40 ml Solution B
  • 50 ml Solution C
  • 450 ml Solution D
  • 26 ml Solution E
  • 10 ml Solution F
  • 1 ml Trace element solution SL-10 B

43 PFENNIG'S MEDIUM I WITH SALT

Compound Conc. [g/L] Conc. [mM]
NaCl 30 513.347
NaHCO3 1.5 17.856
Na2S x 9 H2O 0.593 2.467
MgSO4 x 7 H2O 0.5 2.029
Ammonium chloride 0.35 6.543
Show more
Solutions:
  • 460 ml Solution A
  • 40 ml Sulfide solution, 1.5%
  • 50 ml Bicarbonate solution
  • 450 ml Resazurin solution
  • 26 ml Pfennig's heterotrophic salts solution
  • 1 ml Vitamin B12 solution
  • 10 ml Neutralized sulfide solution 3% (w/v)
  • 1 ml Trace element solution SL-12 B

46 PFENNIG'S MEDIUM I WITH SALT

Compound Conc. [g/L] Conc. [mM]
NaCl 10 171.116
NaHCO3 1.5 17.856
Na2S x 9 H2O 0.593 2.467
MgSO4 x 7 H2O 0.5 2.029
Ammonium chloride 0.35 6.543
Show more
Solutions:
  • 460 ml Solution A
  • 40 ml Sulfide solution, 1.5%
  • 50 ml Bicarbonate solution
  • 450 ml Resazurin solution
  • 26 ml Pfennig's heterotrophic salts solution
  • 1 ml Vitamin B12 solution
  • 10 ml Neutralized sulfide solution 3% (w/v)
  • 1 ml Trace element solution SL-12 B
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