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Current criteria: L-Histidin
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78d MEDIUM FOR TREPONEMA PARVUM
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Ground beef | 500 | - |
| Casitone | 30 | - |
| Agar | 15 | 44.599 |
| K2HPO4 | 5 | 28.707 |
| Glutathione | 1.4 | 4.556 |
| Na-pyruvate | 0.5 | 4.544 |
| L-Cysteine HCl | 0.5 | 3.172 |
| N-Acetylglucosamine | 0.5 | 2.26 |
| Ethanol | 0.19 | 4.124 |
| Casamino acids | 0.16 | - |
| Yeast extract | 0.16 | - |
| Tryptone peptone | 0.16 | - |
| Ascorbic acid | 0.1 | 0.568 |
| L-Glutamine | 0.07 | 0.479 |
| L-Histidin | 0.06 | 0.387 |
| L-Serin | 0.05 | 0.476 |
| Vitamin K3 | 0.05 | - |
| Resazurin | 1.0e-3 | 0.004 |
| Hemin | 5.0e-4 | 7.7e-4 |
| p-Aminobenzoic acid | 2.5e-4 | 0.002 |
| alpha-lipoic acid | 2.5e-4 | 0.001 |
| Pantothenic acid | 2.5e-4 | 0.001 |
| Thiamine-HCl x 2 H2O | 2.5e-4 | 6.7e-4 |
| Riboflavin | 2.5e-4 | 6.6e-4 |
| Vitamin B12 | 2.5e-4 | 1.8e-4 |
| Nicotine amide | 1.3e-4 | 0.001 |
| Nicotinic acid | 1.3e-4 | 0.001 |
| Biotin | 1.0e-4 | 4.1e-4 |
| Folic acid | 1.0e-4 | 2.3e-4 |
| Pyridoxamine hydrochloride | 5.0e-5 | 2.4e-4 |
| Calf serum | - | - |
| Vitamin K1 | - | - |
| KCl | - | - |
| Water | - | - |
| NaOH | - | - |
| Distilled water | - | - |
Solutions:
- 10 ml Haemin solution
- 10 ml Vitamin K3 solution
- 10 ml Vitamin K1 solution
- 5 ml Vitamin solution
- 5 ml Amino acid solution
- 5 ml Peptone mixture
- 5 ml Hemin solution
- 5 ml Ascorbic acid solution 2%(w/v)
- 5 ml N-acetylglucosamin- and pyruvate solution
- 10 ml Glutathione solution 14%(w/v)
- 10 ml Sugar solution
Gas phase:
- 80 % N2
- 20 % CO2