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The medium composition is currently in its beta status, meaning that some concentrations are not correct and need further adjustments. Keep this in mind while trying this out.

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Current criteria: Deionized water

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379 CHROMATIUM TEPIDUM MEDIUM

Compound Conc. [g/L] Conc. [mM]
NaHCO3 2 23.808
Na2S x 9 H2O 1 4.164
NH4Cl 0.4 7.478
NaCl 0.4 6.845
MgSO4 x 7 H2O 0.2 0.811
CaCl2 x 2 H2O 0.05 0.34
Na2-EDTA 0.005 0.015
FeCl2 x 4 H2O 0.002 0.008
Ammonium acetate 5.0e-4 0.006
Na pyruvate 5.0e-4 0.005
KH2PO4 5.0e-4 0.004
CoCl2 x 6 H2O 1.9e-4 0.001
Na2MoO4 x 2 H2O 1.9e-4 7.8e-4
MnCl2 x 4 H2O 1.0e-4 5.1e-4
ZnCl2 7.0e-5 5.1e-4
VOSO4 x 2 H2O 3.0e-5 1.5e-4
NiCl2 x 6 H2O 2.5e-5 1.9e-4
H3BO3 6.0e-6 9.7e-5
CuCl2 x 2 H2O 2.0e-6 1.2e-5
Na2WO4 x 2 H2O 2.0e-6 6.1e-6
Deionized water - -
Solutions:
  • 1 ml Trace element solution

594 THERMOMICROBIUM FOSTERI MEDIUM

Compound Conc. [g/L] Conc. [mM]
Agar 20 59.465
NH4Cl 2 37.39
Na2HPO4 0.21 1.479
MgSO4 x 7 H2O 0.2 0.811
NaH2PO4 0.09 0.75
KCl 0.04 0.537
CaCl2 0.015 0.135
FeSO4 x 7 H2O 1.0e-3 0.004
ZnSO4 x 7 H2O 7.0e-5 2.4e-4
H3BO3 1.0e-5 1.6e-4
MoO3 1.0e-5 6.9e-5
MnSO4 x 5 H2O 1.0e-5 4.1e-5
CuSO4 x 5 H2O 5.0e-6 2.0e-5
Deionized water - -
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687 COLUMBIA BLOOD AGAR

Compound Conc. [g/L] Conc. [mM]
Defibrinated Blood 50 -
Agar 0.66 1.962
Bacto Bitone 0.44 -
Bacto Panton 0.44 -
NaCl 0.22 3.765
Tryptic Digest of beef heart 0.132 -
Corn starch 0.044 -
Deionized water - -
Solutions:
  • 44 ml Columbia Blood Agar Base

1147 RV5 MEDIUM

Compound Conc. [g/L] Conc. [mM]
NaCl 15 256.674
DL-Malic acid 4 29.831
BICINE buffer 1.632 10
K2HPO4 1 5.741
NH4Cl 0.52 9.721
MgSO4 x 7 H2O 0.2 0.811
CaCl2 x 2 H2O 0.075 0.51
EDTA 0.005 0.018
FeCl2 x 4 H2O 0.002 0.008
Thiamine 1.0e-3 0.004
CoCl2 x 6 H2O 1.9e-4 0.001
Na2MoO4 x 2 H2O 1.9e-4 7.8e-4
MnCl2 x 4 H2O 1.0e-4 5.1e-4
ZnCl2 7.0e-5 5.1e-4
VOSO4 x 2 H2O 3.0e-5 1.5e-4
Biotin 3.0e-5 1.2e-4
NiCl2 x 6 H2O 2.5e-5 1.9e-4
Vitamin B12 2.0e-5 1.5e-5
CuCl2 x 2 H2O 1.7e-5 1.0e-4
H3BO3 6.0e-6 9.7e-5
Na2WO4 x 2 H2O 2.0e-6 6.1e-6
NaHSeO3 2.0e-6 -
Deionized water - -
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Solutions:
  • 1 ml Trace elements

1203 FASTIDIOUS ANAEROBE AGAR

Compound Conc. [g/L] Conc. [mM]
Horse blood 100 -
Fastidious Anaerobe Agar 45.7 -
Deionized water - -

1203a FASTIDIOUS ANAEROBE BROTH

Compound Conc. [g/L] Conc. [mM]
Fastidious Anaerobe Basal Broth 35.4 -
Deionized water - -

1344 LYU03 Medium

Compound Conc. [g/L] Conc. [mM]
Taps 17.4 -
NaHCO3 2.5 29.759
Sodium acetate 1 12.191
Disodium succinate 1 6.171
NH4Cl 0.5 9.348
KH2PO4 0.5 3.674
Na2S x 9 H2O 0.45 1.874
MgSO4 x 7 H2O 0.2 0.811
Na2S2O3 x 5 H2O 0.1 0.403
Yeast extract 0.1 -
CaCl2 x 2 H2O 0.075 0.51
EDTA 0.005 0.018
Na2-EDTA 0.005 0.015
FeCl2 x 4 H2O 0.002 0.008
Niacin 5.0e-4 0.004
Thiamine HCl 5.0e-4 0.001
p-Aminobenzoic acid 3.0e-4 0.002
CoCl2 x 6 H2O 1.9e-4 0.001
Na2MoO4 x 2 H2O 1.9e-4 7.8e-4
MnCl2 x 4 H2O 1.0e-4 5.1e-4
Pyridoxamine hydrochloride 1.0e-4 4.9e-4
ZnCl2 7.0e-5 5.1e-4
Biotin 5.0e-5 2.0e-4
Vitamin B12 4.0e-5 3.0e-5
VOSO4 x 2 H2O 3.0e-5 1.5e-4
NiCl2 x 6 H2O 2.5e-5 1.9e-4
CuCl2 x 2 H2O 1.7e-5 1.0e-4
H3BO3 6.0e-6 9.7e-5
Na2WO4 x 2 H2O 2.0e-6 6.1e-6
NaHSeO3 2.0e-6 -
Deionized water - -
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Solutions:
  • 1 ml Trace elements (A)
  • 30 ml Neutralized sulfide solution (B)
  • 30 ml Bicarbonate solution (C)
  • 1 ml Vitamin solution (D)

1381 ½ S° HELIORESTIS MEDIUM

Compound Conc. [g/L] Conc. [mM]
NaHCO3 2.5 29.759
Na2CO3 2.5 23.588
Bicine 1.63 9.99
Na-acetate 1 12.19
Na-pyruvate 1 9.087
NH4Cl 0.5 9.348
KH2PO4 0.5 3.674
Na2S x 9 H2O 0.5 2.082
Na2S2O3 x 5 H2O 0.5 2.015
MgSO4 x 7 H2O 0.2 0.811
Yeast extract 0.1 -
CaCl2 0.075 0.676
EDTA 0.005 0.018
FeCl2 x 4 H2O 0.002 0.008
CoCl2 x 6 H2O 1.9e-4 0.001
Na2MoO4 x 2 H2O 1.9e-4 7.8e-4
MnCl2 x 4 H2O 1.0e-4 5.1e-4
ZnCl2 7.0e-5 5.1e-4
VOSO4 x 2 H2O 3.0e-5 1.5e-4
NiCl2 x 6 H2O 2.5e-5 1.9e-4
Vitamin B12 2.0e-5 1.5e-5
CuCl2 x 2 H2O 1.7e-5 1.0e-4
H3BO3 6.0e-6 9.7e-5
Na2WO4 x 2 H2O 2.0e-6 6.1e-6
NaHSeO3 2.0e-6 -
Deionized water - -
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Solutions:
  • 1 ml Trace elements

1469 THERMOCHROMATIUM/ALLOCHROMATIUM MEDIUM

Compound Conc. [g/L] Conc. [mM]
MOPS 1.225 5.854
Na acetate 1.167 14.222
NaHCO3 1 11.904
Na2S x 9 H2O 0.75 3.123
KH2PO4 0.583 4.287
Sodium pyruvate 0.5 4.544
NH4Cl 0.467 8.724
NaCl 0.467 7.985
MgSO4 x 7 H2O 0.233 0.947
Yeast extract 0.1 -
CaCl2 x 2 H2O 0.088 0.595
EDTA 0.006 0.021
FeCl2 x 4 H2O 0.002 0.009
CoCl2 x 6 H2O 2.2e-4 0.002
Na2MoO4 x 2 H2O 2.2e-4 9.1e-4
MnCl2 x 4 H2O 1.2e-4 5.9e-4
ZnCl2 8.2e-5 6.0e-4
VOSO4 x 2 H2O 3.5e-5 1.8e-4
NiCl2 x 6 H2O 2.9e-5 2.3e-4
CuCl2 x 2 H2O 2.0e-5 1.2e-4
H3BO3 7.0e-6 1.1e-4
Na2WO4 x 2 H2O 2.3e-6 7.1e-6
NaHSeO3 2.3e-6 -
Double distilled water - -
Vitamine B12 - -
Deionized water - -
Solutions:
  • 700 ml Solution 1 Mineral Salts
  • 300 ml Solution 2 NaHCO3
  • 1 ml Trace elements

1582 MELISSOCOCCUS PLUTONIUS MEDIUM

Compound Conc. [g/L] Conc. [mM]
KH2PO4 13.5 99.203
Glucose 10 55.506
Starch 10 -
Yeast extract 10 -
Cysteine hydrochloride 1 6.344
Deionized water - -
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