Main sol. 1814
Internal Solution-ID: S6324
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Na2CO3 | 15 | g/l | 15 | 141.525 | |
| NaHCO3 | 20 | g/l | 20 | 238.075 | |
| 1 Autoclave carbonate and bicarbonate powder (dry) together in a 1 L bottle. | |||||
| 2 Autoclave at 121 °C for 15 min. | |||||
| 3 Dissolve the following compounds in 1 L and autoclave separately. | |||||
| NaCl | 3 | g/l | 3 | 51.335 | |
| K2HPO4 | 1 | g/l | 1 | 5.741 | |
| Trace element solution | 1 | ml | - | - | |
| Distilled water | 1000 | ml | - | - | |
| 4 Autoclave at 121 °C for 15 min. | |||||
| 5 Combine the autoclaved solution with the autoclaved carbonate and bicarbonate powder and dissolve. | |||||
| 6 After combining the autoclaved compounds add the following sterile solutions. | |||||
|
MgSO4
(1M in water, autoclaved) |
1 | ml | 0.12 | 1 | |
|
Yeast extract
(1% in water, autoclaved) |
2 | ml | 0.02 | - | |
|
NH4Cl
(2M in water, autoklaved) |
2 | ml | 0.214 | 4 | |
|
Cellobiose
(10% solution in water, autoklaved) |
10 | ml | 1 | 2.921 | |
| Vitamine mixture | 2 | ml | - | - | |