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Trace element solution

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Internal Solution-ID: S3027

This solution is used in 6 media and was first defined in medium 1478.

Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
EDTA 5.00 g 5 17.109
FeSO4 x 7 H2O 2.00 g 2 7.194
ZnSO4 0.10 g 0.1 0.62
MnCl2 0.03 g 0.03 0.238
H3BO3 0.30 g 0.3 4.851
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 x 5 H2O 0.01 g 0.01 0.045
NiCl2 x 6 H2O 0.02 g 0.02 0.154
Na2MoO4 x 2 H2O 0.02 g 0.02 0.083
Distilled water 1000.00 ml - -
1 Adjust pH to 3.0-4.0 with HCl.
2 Autoclave at 121 °C for 15 min.
3 Note: autoclave in closed bottles
1478 NATRANAEROARCHAEUM MEDIUM AARC1
1479 HALODESULFURIARCHAEUM MEDIUM
1491 CYCLONATRONUM PROTEIVORANS MEDIUM (OMEGA)
1615 Basic mineral medium
1778a ANB aerobic
1840 Halorutilales haloarchaeon medium