Main sol. 473
Internal Solution-ID: S972
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| K2HPO4 | 1.400 | g | 1.4 | 8.038 | |
| KH2PO4 | 0.272 | g | 0.272 | 1.999 | |
| Ca(NO3)2 x 4 H2O | 0.020 | g | 0.02 | 0.085 | |
| MgSO4 x 7 H2O | 0.490 | g | 0.49 | 1.988 | |
| Ferric ammonium citrate | 0.080 | g | 0.08 | - | |
| Na2-EDTA | 0.200 | g | 0.2 | 0.595 | |
| Agar | 15.000 | g | 15 | 44.599 | |
| Trace element solution SL-6 | 5.000 | ml | - | - | |
| Distilled water | 1000.000 | ml | - | - | |
| Vitamin solution | 5.000 | ml | - | - | |
| 1 Autoclave phosphate buffer separately. pH should be 7.5 - 7.6. Add 5.0 ml sterile stock solution (see medium 462). | |||||
Media with this solution
| 473 | MEDIUM WITH EDTA AS CARBON SOURCE |