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DSMZ

DSMZ 473 . MEDIUM WITH EDTA AS CARBON SOURCE

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 473 #

ml
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
K2HPO4 1.400 g 1.4 8.038
KH2PO4 0.272 g 0.272 1.999
Ca(NO3)2 x 4 H2O 0.020 g 0.02 0.085
MgSO4 x 7 H2O 0.490 g 0.49 1.988
Ferric ammonium citrate 0.080 g 0.08 -
Na2-EDTA 0.200 g 0.2 0.595
Agar 15.000 g 15 44.599
Trace element solution SL-6 5.000 ml - -
Distilled water 1000.000 ml - -
Vitamin solution 5.000 ml - -
1 Autoclave phosphate buffer separately. pH should be 7.5 - 7.6. Add 5.0 ml sterile stock solution (see medium 462).

DSM 6780 Vitamin solution #

100
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 0.08 g 0.8 3.275
Folic acid 0.08 g 0.8 1.812
Thiamine HCl 0.08 g 0.8 2.372
Distilled water 100.00 ml - -

Trace element solution SL-6 #

1000
Solution occurs in 55 other media (first defined in Medium 27).
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
MnCl2 x 4 H2O 0.03 g 0.03 0.152
H3BO3 0.30 g 0.3 4.851
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 x 2 H2O 0.01 g 0.01 0.059
NiCl2 x 6 H2O 0.02 g 0.02 0.154
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
Distilled water 1000.00 ml - -

Vitamin solution #

100
Solution occurs in 4 other media (first defined in Medium 462).
Compound Amount Unit Conc. [g/L] Conc. [mM]
p-Aminobenzoate 1.0 mg 0.01 0.073
Biotin 0.2 mg 0.002 0.008
Nicotinic acid 2.0 mg 0.02 0.162
Thiamine-HCl x 2 H2O 1.0 mg 0.01 0.027
Calcium pantothenate 0.5 mg 0.005 0.01
Pyridoxamine 5.0 mg 0.05 0.297
Vitamin B12 2.0 mg 0.02 0.015
Distilled water 100.0 ml - -

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.5 - 7.6
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 8
Strains with modifications: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Agar 15 44.599
K2HPO4 1.4 8.038
MgSO4 x 7 H2O 0.49 1.988
KH2PO4 0.272 1.999
Na2-EDTA 0.2 0.595
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Cultivation metadata from 8 strains BacDive

pH 6 - 8 1 strain
phyla 1 8 strains
oxygen aerobe 1 strain
temperature mesophilic 8 strains