Main sol. 31
Internal Solution-ID: S5552
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Peptone | 5 | g | 5 | - | |
| Meat extract | 3 | g | 3 | - | |
| Agar , if necessary | 15 | g | 15 | 44.599 | |
| Distilled water | 900 | ml | - | - | |
| 1 After sterilization add sterile 1 M Na-sesquicarbonate solution (1 ml in 10 ml) to achieve a pH of 9.7 | |||||
| Na-sesquicarbonate solution | 100 | ml | - | - | |
| 2 For Bacillus strains the addition of 10.0 mg MnSO4 x H2O is recommended for sporulation. | |||||
Media with this solution
| 31 | ALKALINE NUTRIENT AGAR |