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Main sol. 219

Internal Solution-ID: S476

Compound Amount Unit Conc. [g/L] Conc. [mM]
Yeast extract 2.0 g 2 -
Proteose peptone no. 3 2.0 g 2 -
Casein peptone
(tryptic digested)
2.0 g 2 -
Na2HPO4 x 12 H2O 2.5 g 2.5 17.611
KH2PO4 1.0 g 1 7.348
Sodium citrate 1.5 g 1.5 5.812
MgSO4 x 7 H2O 0.6 g 0.6 2.434
Glycerol 50.0 ml - -
Tween 0.5 g 0.5 -
Agar 20.0 g 20 -
Distilled water 1000.0 ml - -
1 Adjust pH to 7.0.
219 MYCOBACTERIUM MEDIUM