Main sol. J182
Internal Solution-ID: S3840
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Glucose | 10.0 | g | 11.111 | 61.674 | |
|
Polypeptone
(Nihon Pharm. Co.) |
5.0 | g | 5.556 | - | |
| Yeast extract | 5.0 | g | 5.556 | - | |
| NaCl | 10.0 | g | 11.111 | 190.129 | |
| K2HPO4 | 1.0 | g | 1.111 | 6.379 | |
| MgSO4 x 7 H2O | 0.2 | g | 0.222 | 0.902 | |
| Agar | 15.0 | g | 16.667 | 49.555 | |
| Distilled water | 900.0 | ml | - | - | |
| 1 After autoclaving, aseptically add 100 ml of a sterile 10% NaHCO3 solution to the medium. Check final pH to be about 9.0. | |||||
Media with this solution
| J182 | MODIFIED HORIKOSHI-I MEDIUM |