Main sol. J14
Internal Solution-ID: S3635
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Lab-Lemco beef extract
(Oxoid) |
2.4 | g | 2.4 | - | |
|
Proteose peptone no. 3
(BD-Difco) |
10.0 | g | 10 | - | |
|
Yeast extract
(BD-Difco) |
5.0 | g | 5 | - | |
| Na2HPO4 | 4.0 | g | 4 | 28.177 | |
| Glucose | 1.5 | g | 1.5 | 8.326 | |
|
Starch
(soluble) |
0.5 | g | 0.5 | - | |
| L-Cystine | 0.2 | g | 0.2 | 0.832 | |
| Agar | 15.0 | g | 15 | 44.599 | |
| L-Cysteine HCl x H2O | 0.5 | g | 0.5 | - | |
| Horse blood | 50.0 | ml | - | - | |
| Distilled water | 950.0 | ml | - | - | |
| 1 Add L-cystine to 50 ml of 1 N HCl and mix thoroughly. Add the remaining components and bring volume to 950 ml. Adjust pH to 7.6-7.8. After autoclaving and then cooling to 50C, aseptically add 50.0 ml of horse blood. Mix thoroughly and pour into sterile petri dishes or distribute into sterile tubes. | |||||
Media with this solution
| J14 | EG MEDIUM |