Main sol. 1569
Internal Solution-ID: S3253
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 120.0 | g | 120 | 2053.39 | |
| K2HPO4 | 2.5 | g | 2.5 | 14.353 | |
| (NH4)2SO4 | 0.5 | g | 0.5 | 3.784 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Adjust pH to 7.0 with KH2PO4 or K2HPO4. Make anoxic under nitrogen stream and autoclave at 121°C. | |||||
| 2 After autoclaving add sterile, anoxic solutions of: | |||||
|
Chitin
, adjust in range from 1 - 2 if required
(amorphous, from 5 % solution) |
1.5 | g/l | 1.5 | - | |
| MgCl2 x 6 H2O | 5.0 | mM | 1.017 | 5 | |
| Yeast extract | 20.0 | mg/l | 0.02 | - | |
| NaHCO3 | 20.0 | mM | 1.68 | 20 | |
| Na2S | 1.0 | mM | 0.079 | 1 | |
| Acidic trace metals (Pfennig & Lippert, 1965) | 1.0 | ml/l | - | - | |
| Vitamine mixture (Pfennig & Lippert, 1965) | 1.0 | ml/l | - | - | |
Media with this solution
| 1569 | ORENIA CHITINITROPHA |