Equipment needed: Autoclave
Hungate tubes
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Glucose | 1.0 | g | 1 | 5.551 | |
|
Trypticase
(BBL) |
5.0 | g | 5 | - | |
| KH2PO4 | 1.0 | g | 1 | 7.348 | |
| Na-acetate | 4.0 | g | 4 | 48.76 | |
| Yeast extract | 2.0 | g | 2 | - | |
| n-Valeric acid | 0.1 | ml | - | - | |
| Resazurin | 1.0 | mg | 1.0e-3 | 0.004 | |
| Na2CO3 | 4.0 | g | 4 | 37.74 | |
| L-Cysteine HCl x H2O | 0.5 | g | 0.5 | 2.847 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Adjust pH to 7.0. Gas atmosphere: 100% CO2. | |||||
| 2 Distribute into Hungate tubes and autoclave. | |||||
Media with this solution
| 181 | SELENOMONAS RUMINANTIUM MEDIUM |