Main sol. 153
Internal Solution-ID: S268
Equipment needed: Autoclave
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Trypticase
(BBL) |
15.0 | g | 15 | - | |
| L-Cystine | 0.5 | g | 0.5 | 2.081 | |
| Glucose | 2.0 | g | 2 | 11.101 | |
| Yeast extract | 5.0 | g | 5 | - | |
| NaCl | 2.5 | g | 2.5 | 42.779 | |
| Na-thioglycolate | 0.5 | g | 0.5 | 4.382 | |
|
Sodium resazurin
(0.1% w/v) |
0.5 | ml | 5.0e-4 | 0.002 | |
|
Agar
, for solid medium
optional |
15.0 | g | 15 | 44.599 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Dissolve ingredients, sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Adjust pH to 7.1 with Na2CO3 (5% w/v), if necessary. Then dispense medium under the same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. | |||||
Media with this solution
| 153 | THIOGLYCOLATE MEDIUM (MERCK 108190) |