Main sol. 802
Internal Solution-ID: S1632
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 100.0 | g/l | 100 | 1711.16 | |
| MgSO4 x 7 H2O | 20.0 | g/l | 20 | 81.144 | |
| KCl | 2.0 | g/l | 2 | 26.828 | |
| NaBr | 0.1 | g/l | 0.1 | 0.972 | |
|
Yeast extract
(Difco) |
2.0 | g/l | 2 | - | |
|
Trypticase peptone
(BBL) |
2.0 | g/l | 2 | - | |
| Tris-HCl | 12.0 | g/l | 12 | - | |
| Trace metals solution | 2.0 | ml | - | - | |
| Distilled water | 998.0 | ml | - | - | |
| 1 Adjust pH to 7.8 (with NaOH) before autoclaving. To the sterile medium, from sterile stock solutions, add 10 ml KH2PO4 (50 g/l), 5 ml CaCl2 (100 g/l), 2 ml FeCl2/MnCl2 (FeCl2 x 4 H2O, 20 g/l and MnCl2 x 4 H2O, 20 g/l), and glucose to a final concentration of 2 g/l. | |||||
|
KH2PO4
(50 g/l) |
10.0 | ml | 23.4 | 171.951 | |
|
CaCl2
(100 g/l) |
5.0 | ml | - | - | |
| FeCl2/MnCl2 solution | 2.0 | ml | - | - | |
| Glucose | 2.0 | g | 2 | 11.101 | |
Media with this solution
| 802 | GRACILIBACILLUS MEDIUM |