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Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
NaCl | 100.0 | g/l | 100 | 1711.16 | |
MgSO4 x 7 H2O | 20.0 | g/l | 20 | 81.144 | |
KCl | 2.0 | g/l | 2 | 26.828 | |
NaBr | 0.1 | g/l | 0.1 | 0.972 | |
Yeast extract
(Difco) |
2.0 | g/l | 2 | - | |
Trypticase peptone
(BBL) |
2.0 | g/l | 2 | - | |
Tris-HCl | 12.0 | g/l | 12 | - | |
Trace metals solution | 2.0 | ml | - | - | |
Distilled water | 998.0 | ml | - | - | |
1 Adjust pH to 7.8 (with NaOH) before autoclaving. To the sterile medium, from sterile stock solutions, add 10 ml KH2PO4 (50 g/l), 5 ml CaCl2 (100 g/l), 2 ml FeCl2/MnCl2 (FeCl2 x 4 H2O, 20 g/l and MnCl2 x 4 H2O, 20 g/l), and glucose to a final concentration of 2 g/l. | |||||
KH2PO4
(50 g/l) |
10.0 | ml | 23.4 | 171.951 | |
CaCl2
(100 g/l) |
5.0 | ml | - | - | |
FeCl2/MnCl2 solution | 2.0 | ml | - | - | |
Glucose | 2.0 | g | 2 | 11.101 |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
HCl
(32%) |
10 | ml | 3.2 | 87.767 | |
FeCl2 x 4 H2O | 2 | g | 1.98 | 9.96 | |
CoCl2 x 6 H2O | 250 | mg | 0.248 | 1.906 | |
MnCl2 x 4 H2O | 100 | mg | 0.099 | 0.5 | |
ZnCl2 | 70 | mg | 0.069 | 0.508 | |
H3BO3 | 6 | mg | 0.006 | 0.096 | |
Na2MoO4 x 2 H2O | 40 | mg | 0.04 | 0.164 | |
NiCl2 x 6 H2O | 70 | mg | 0.069 | 0.535 | |
CuCl2 x 2 H2O | 2 | mg | 0.002 | 0.012 | |
AlCl3 x 6 H2O | 60 | mg | 0.059 | 0.246 | |
Na2WO4 x 2 H2O | 6 | mg | 0.006 | 0.018 | |
Distilled water | 1000 | ml | - | - | |
1 Dissolve salts in the HCl in the oder given above, then make up the solution to 1 litre with distilled water. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
FeCl2 x 4 H2O | 20 | g | 20 | 100.598 |
MnCl2 x 4 H2O | 20 | g | 20 | 101.059 |
Last modified: | 22.02.22 |
Source: | DSMZ |
Taxonomic range: | Bacteria |
Medium type: | Complex medium |
Final pH: | 7.8 |
Edit in Medium builder |
All strains for this medium: | 2 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
NaCl | 100 | 1711.16 |
KH2PO4 | 23.4 | 171.951 |
MgSO4 x 7 H2O | 20 | 81.144 |
Tris-HCl | 12 | - |
KCl | 2 | 26.828 |
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