Equipment needed: Hungate tubes
Gassing station
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Casein peptone
(tryptic digest) |
10 | g | 10 | - | |
| Yeast extract | 5 | g | 5 | - | |
| Na-lactate | 10 | g | 10 | 89.238 | |
| Agar | 15 | g | 15 | 44.599 | |
| Distilled water | 1000 | ml | - | - | |
| 1 Adjust pH to 7.0 - 7.2. For anaerobic handling of the liquid medium (without agar), prepare and distribute into Hungate tubes by use of the Hungate technique. | |||||
Media with this solution
| 91 | PROPIONIBACTERIUM AGAR |