J990
990 .
MODIFIED MPOB MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Medium for Microbacter margulisiae DSM 27471
This strain uses the standard medium without modifications.
Main sol. J990 #
ml
Equipment needed: Anaerobic workbench
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Na2HPO4 | 0.53 | g | 0.524 | 3.693 | |
| KH2PO4 | 0.41 | g | 0.406 | 2.98 | |
| NH4Cl | 0.30 | g | 0.297 | 5.548 | |
| CaCl2 x 2 H2O | 0.11 | g | 0.109 | 0.74 | |
| MgCl2 x 6 H2O | 0.10 | g | 0.099 | 0.487 | |
| NaCl | 0.30 | g | 0.297 | 5.078 | |
| Trace element solution SL-10 | 1.00 | ml | - | - | |
|
Yeast extract
(BD-Difco) |
0.40 | g | 0.396 | - | |
| Resazurin | 0.50 | mg | 4.9e-4 | 0.002 | |
| Trace vitamins | 10.00 | ml | - | - | |
| Glucose | 1.80 | g | 1.78 | 9.882 | |
| L-Cysteine HCl x H2O | 0.50 | g | 0.495 | - | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Prepare the medium anaerobically under a N2-CO2 (4:1, v/v) gas mixture. Mix ingredients except Trace vitamins. After autoclaving, anaerobically and aseptically add the filter-sterilized Trace vitamins to the medium. Check final pH to be 7.0 - 7.2. Pressurize the inoculated bottles to 70 kPa N2-CO2 (4:1, v/v). | |||||
Solution occurs in 20
other media (first defined in Medium J433).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
HCl
(25%, 7.7 M) |
10.0 | ml | 2.5 | 68.568 | |
| FeCl2 x 4 H2O | 1.5 | g | 1.5 | 7.545 | |
| ZnCl2 | 70.0 | mg | 0.07 | 0.514 | |
| MnCl2 x 4 H2O | 100.0 | mg | 0.1 | 0.505 | |
| H3BO3 | 6.0 | mg | 0.006 | 0.097 | |
| CoCl2 x 6 H2O | 190.0 | mg | 0.19 | 1.463 | |
| CuCl2 x 2 H2O | 2.0 | mg | 0.002 | 0.012 | |
| NiCl2 x 6 H2O | 24.0 | mg | 0.024 | 0.185 | |
| Na2MoO4 x 2 H2O | 36.0 | mg | 0.036 | 0.149 | |
| Distilled water | 990.0 | ml | - | - | |
| 1 Dissolve FeCl2 x 4H2O first in HCl. Then dilute in distilled water and add the remaining salts. | |||||
Solution occurs in 220
other media (first defined in Medium J197).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Biotin | 2.0 | mg | 0.002 | 0.008 |
| Folic acid | 2.0 | mg | 0.002 | 0.005 |
| Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
| Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
| Riboflavin | 5.0 | mg | 0.005 | 0.013 |
| Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
| Calcium pantothenate | 5.0 | mg | 0.005 | 0.01 |
| Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
| p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
| Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
| Distilled water | 1000.0 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.1 |
| Equipment needed: | Anaerobic workbench, Filter |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 2 |
Microbacter margulisiae DSM 27471
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Glucose | 1.78 | 9.882 |
| Na2HPO4 | 0.524 | 3.693 |
| L-Cysteine HCl x H2O | 0.495 | - |
| KH2PO4 | 0.406 | 2.98 |
| Yeast extract | 0.396 | - |
| Show more | ||
