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Main sol. J990

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Internal Solution-ID: S5020

Equipment needed: Anaerobic workbench Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2HPO4 0.53 g 0.524 3.693
KH2PO4 0.41 g 0.406 2.98
NH4Cl 0.30 g 0.297 5.548
CaCl2 x 2 H2O 0.11 g 0.109 0.74
MgCl2 x 6 H2O 0.10 g 0.099 0.487
NaCl 0.30 g 0.297 5.078
Trace element solution SL-10 1.00 ml - -
Yeast extract
(BD-Difco) 		0.40 g 0.396 -
Resazurin 0.50 mg 4.9e-4 0.002
Trace vitamins 10.00 ml - -
Glucose 1.80 g 1.78 9.882
L-Cysteine HCl x H2O 0.50 g 0.495 -
Distilled water 1000.00 ml - -
1 Prepare the medium anaerobically under a N2-CO2 (4:1, v/v) gas mixture. Mix ingredients except Trace vitamins. After autoclaving, anaerobically and aseptically add the filter-sterilized Trace vitamins to the medium. Check final pH to be 7.0 - 7.2. Pressurize the inoculated bottles to 70 kPa N2-CO2 (4:1, v/v).
J990 MODIFIED MPOB MEDIUM