J692
692 .
ANAEROBIC SULFOLOBALES MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J692 #
ml
Equipment needed: Autoclave
Anaerobic workbench
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Salt base solution | 1000.0 | ml | - | - | |
|
Yeast extract
(BD-Difco) |
0.5 | g | 0.5 | - | |
| Resazurin | 1.0 | mg | 1.0e-3 | 0.004 | |
| Na2S x 9 H2O | 0.5 | g | 0.5 | 2.082 | |
|
Sulfur
(powder) |
1.0 | g | 1 | 31.182 | |
| 1 Autoclave Salt base solution under a H2-CO2 (4:1, v/v) gas mixture. Separately autoclave yeast extract (as a 10% solution) under a N2 atmosphere and add to the Salt base solution. Steam sulfur for 3 hr on each of 3 successive days. Distribute the medium into culture vessels containing an appropriate amount of sulfur (e.g., 20 ml medium and 0.02 g sulfur in 120 ml serum bottle) under a stream of H2-CO2 (4:1, v/v) and seal with butyl rubber stoppers. Add Na2S x 9H2O from anaerobic, sterile stock solution (5%, autoclaved under a N2 atmosphere) and readjust pH to 3.0. Pressurize the inoculated culture vessels to 100 kPa with the same gas mixture. | |||||
Solution occurs in 1
other media (first defined in Medium J691).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| (NH4)2SO4 | 1.30 | g | 1.3 | 9.838 | |
| KH2PO4 | 0.28 | g | 0.28 | 2.058 | |
| MgSO4 x 7 H2O | 0.25 | g | 0.25 | 1.014 | |
| CaCl2 x 2 H2O | 0.07 | g | 0.07 | 0.476 | |
| MnCl2 x 4 H2O | 1.80 | mg | 0.002 | - | |
| Na2B4O7 x 10 H2O | 4.50 | mg | 0.005 | - | |
| ZnSO4 x 7 H2O | 0.22 | mg | 2.2e-4 | 7.7e-4 | |
| CuCl2 x 2 H2O | 0.05 | mg | 5.0e-5 | 2.9e-4 | |
| Na2MoO4 x 2 H2O | 0.03 | mg | 3.0e-5 | 1.2e-4 | |
| VOSO4 x n H2O | 0.03 | mg | 3.0e-5 | - | |
| CoSO4 x 7 H2O | 0.01 | mg | 1.0e-5 | 6.5e-5 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Adjust pH to 2.5 with 10 N H2SO4. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Medium type: | Complex medium |
| Final pH: | 3.0 |
| Equipment needed: | Anaerobic workbench, Autoclave |
| Edit in Medium builder | |