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JCM 669 . MAGNETOSPIRILLUM MEDIUM (B)

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
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Medium for Paramagnetospirillum magnetotacticum DSM 3856

This strain uses the standard medium without modifications.

Main sol. J669 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.28 g 0.276 2.031
NaNO3 0.12 g 0.118 1.394
Sodium thioglycolate 0.05 g 0.049 0.433
Sodium tartrate 0.37 g 0.365 1.882
Sodium succinate 0.37 g 0.365 2.254
Sodium acetate 0.05 g 0.049 0.602
Trace vitamins 10.00 ml - -
Ferric quinate solution 2.00 ml - -
Mineral salt solution 1.00 ml - -
Distilled water 1000.00 ml - -
1 Adjust pH to 6.75 with NaOH.

Ferric quinate solution #

100
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
FeCl3 x 6 H2O 0.45 g 4.5 16.648
Quinic acid 0.19 g 1.9 9.887
Distilled water 100.00 ml - -
1 Dissolve and autoclave at 121C for 15 min.
2 Comment: For strain JCM 21281, good growth can be obtained in semisolid medium (1.0 g/L agar).

Trace vitamins #

1000
Solution occurs in 220 other media (first defined in Medium J197).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine HCl 5.0 mg 0.005 0.015
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Mineral salt solution #

1000
Solution occurs in 3 other media (first defined in Medium J304).
Compound Amount Unit Conc. [g/L] Conc. [mM]
MgSO4 x 7 H2O 29.70 g 29.7 120.499
Nitrilotriacetic acid 10.00 g 10 52.318
CaCl2 x 2 H2O 3.34 g 3.34 22.719
FeSO4 x 7 H2O 99.00 mg 0.099 0.356
Na2MoO4 x 2 H2O 13.00 mg 0.013 0.054
Metals ''44'' 50.00 ml - -
Distilled water 950.00 ml - -
1 Add nitrilotriacetic acid to 500 ml of distilled water. Dissolve by adjusting pH to 6.5 with KOH. Add the remaining components and distilled water. Readjust pH to 7.2.

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 6.7
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 1
Paramagnetospirillum magnetotacticum DSM 3856

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Sodium succinate 0.365 2.254
Sodium tartrate 0.365 1.882
KH2PO4 0.276 2.031
NaNO3 0.118 1.394
Sodium acetate 0.049 0.602
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen microaerophile 1 strain
temperature mesophilic 1 strain