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JCM 304 . PYGV MARINE MEDIUM (A)

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J304 #

ml
Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Bacto peptone
(BD-Difco) 		0.25 g 0.25 -
Yeast extract
(BD-Difco) 		0.25 g 0.25 -
Mineral salt solution 20.00 ml - -
Glucose
(2.5%) 		10.00 ml - -
Vitamin solution 10.00 ml - -
Agar 15.00 g 15 44.599
Artificial seawater 960.00 ml - -
1 Add components except the glucose solution and Vitamin solution, to artificial seawater and mix thoroughly. Gently heat and bring to a boil. Autoclave and then cool to 45 - 50C. Aseptically add the filter-sterilized glucose solution and Vitamin solution. Adjust pH to 7.5 with sterile KOH, if necessary.

Mineral salt solution #

1000
Solution occurs in 3 other media (first defined in Medium J304).
Compound Amount Unit Conc. [g/L] Conc. [mM]
MgSO4 x 7 H2O 29.70 g 29.7 120.499
Nitrilotriacetic acid 10.00 g 10 52.318
CaCl2 x 2 H2O 3.34 g 3.34 22.719
FeSO4 x 7 H2O 99.00 mg 0.099 0.356
Na2MoO4 x 2 H2O 13.00 mg 0.013 0.054
Metals ''44'' 50.00 ml - -
Distilled water 950.00 ml - -
1 Add nitrilotriacetic acid to 500 ml of distilled water. Dissolve by adjusting pH to 6.5 with KOH. Add the remaining components and distilled water. Readjust pH to 7.2.

Vitamin solution #

1000
Solution occurs in 2 other media (first defined in Medium J304).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 -
Riboflavin 5.0 mg 0.005 0.013
Thiamine HCl 5.0 mg 0.005 -
Nicotinamide 5.0 mg 0.005 0.041
Calcium pantothenate 5.0 mg 0.005 0.01
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Distilled water 1000.0 ml - -

Artificial seawater #

1000
Solution occurs in 1 other media (first defined in Medium J304).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 23.477 g 23.477 401.728
Na2SO4 3.917 g 3.917 27.577
MgCl2 x 6 H2O 4.981 g 4.981 24.5
CaCl2 x 2 H2O 1.102 g 1.102 7.496
NaHCO3 192.000 mg 0.192 2.286
KCl 664.000 mg 0.664 8.907
KBr 6.000 mg 0.006 0.05
H3BO3 26.000 mg 0.026 0.42
SrCl2 x 6 H2O 24.000 mg 0.024 0.09
NaF 3.000 mg 0.003 0.071
Distilled water 1000.000 ml - -

Metals ''44'' #

1000
Solution occurs in 7 other media (first defined in Medium J149).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-EDTA 250.0 mg 0.25 -
ZnSO4 x 7 H2O 1095.0 mg 1.095 3.808
FeSO4 x 7 H2O 500.0 mg 0.5 1.798
MnSO4 x n H2O 154.0 mg 0.154 -
CuSO4 x 5 H2O 39.2 mg 0.039 0.157
Co(NO3)2 x 6 H2O 24.8 mg 0.025 0.085
Na2B4O7 x 10 H2O 17.7 mg 0.018 -
Distilled water 1000.0 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.2
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 22.538 385.659
Agar 15 44.599
MgCl2 x 6 H2O 4.782 23.52
Na2SO4 3.76 26.474
CaCl2 x 2 H2O 1.125 7.65
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen aerobe 1 strain
oxygen obligate aerobe 1 strain
temperature mesophilic 1 strain
temperature psychrophilic 1 strain