# J669: MAGNETOSPIRILLUM MEDIUM (B)


## Main sol. J669

0.28	g	KH2PO4	
0.12	g	NaNO3	
0.05	g	Sodium thioglycolate	
0.37	g	Sodium tartrate	
0.37	g	Sodium succinate	
0.05	g	Sodium acetate	
10	ml	**Trace vitamins**	
2	ml	**Ferric quinate solution**	
1	ml	**Mineral salt solution**	
1000	ml	Distilled water	

1. Adjust pH to 6.75 with NaOH.


## Ferric quinate solution

0.45	g	FeCl3 x 6 H2O	
0.19	g	Quinic acid	
100	ml	Distilled water	

1. Dissolve and autoclave at 121C for 15 min.
2. Comment: For strain JCM 21281, good growth can be obtained in semisolid medium (1.0 g/L agar).


## Trace vitamins

2	mg	Biotin	
2	mg	Folic acid	
10	mg	Pyridoxine hydrochloride	
5	mg	Thiamine HCl	
5	mg	Riboflavin	
5	mg	Nicotinic acid	
5	mg	Calcium pantothenate	
0.1	mg	Vitamin B12	
5	mg	p-Aminobenzoic acid	
5	mg	Lipoic acid	
1000	ml	Distilled water	


## Mineral salt solution

29.7	g	MgSO4 x 7 H2O	
10	g	Nitrilotriacetic acid	
3.34	g	CaCl2 x 2 H2O	
99	mg	FeSO4 x 7 H2O	
13	mg	Na2MoO4 x 2 H2O	
50	ml	**Metals ''44''**	
950	ml	Distilled water	

1. Add nitrilotriacetic acid to 500 ml of distilled water. Dissolve by adjusting pH to 6.5 with KOH. Add the remaining components and distilled water. Readjust pH to 7.2.