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DSMZ

JCM 303 . PE MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×
Medium for Tepidimonas sp. DSM 14591

This strain uses the standard medium without modifications.

Main sol. J303 #

ml
Solution occurs in 2 other media (first defined in Medium J303).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Basal salts solution 5.0 ml - -
Phosphate solution 5.0 ml - -
Vitamin solution 1.0 ml - -
(NH4)2SO4 0.5 g 0.495 3.743
Na glutamate 0.5 g 0.495 2.588
Sodium succinate 0.5 g 0.495 3.052
Sodium acetate 0.5 g 0.495 6.029
Yeast extract
(BD-Difco) 		0.5 g 0.495 -
Casamino acids
(BD-Difco) 		0.5 g 0.495 -
Na2S2O3 0.5 g 0.495 3.128
Distilled water 1000.0 ml - -
1 Adjust pH to 7.5.

Basal salts solution #

1010
Solution occurs in 3 other media (first defined in Medium J303).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na3-EDTA 4.12 g 4.079 -
FeSO4 x H2O 1.11 g 1.099 6.468
MgSO4 x 7 H2O 24.65 g 24.406 99.02
CaCl2 x 2 H2O 2.94 g 2.911 19.8
NaCl 23.40 g 23.168 396.446
Trace elements solution 10.00 ml - -
Distilled water 1000.00 ml - -

Phosphate solution #

1000
Solution occurs in 4 other media (first defined in Medium J303).
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 75 g 75 551.126
K2HPO4 78 g 78 447.823
Distilled water 1000 ml - -

Vitamin solution #

100
Solution occurs in 3 other media (first defined in Medium J303).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nicotinic acid 100 mg 1 8.123
Thiamine HCl 100 mg 1 -
Biotin 5 mg 0.05 0.205
p-Aminobenzoic acid 50 mg 0.5 3.646
Vitamin B12 1 mg 0.01 0.007
Calcium pantothenate 50 mg 0.5 1.049
Pyridoxine hydrochloride 50 mg 0.5 -
Folic acid 50 mg 0.5 1.133
Na3-EDTA 200 mg 2 -
Distilled water 100 ml - -

Trace elements solution #

500
Compound Amount Unit Conc. [g/L] Conc. [mM]
MnSO4 x n H2O 5.58 g 11.16 -
ZnSO4 x 7 H2O 1.44 g 2.88 10.015
Co(NO3)2 x 6 H2O 1.46 g 2.92 10.033
CuSO4 x 5 H2O 1.26 g 2.52 10.093
Na2MoO4 x 2 H2O 1.21 g 2.42 10.002
H3BO3 1.55 g 3.1 50.129
Na3-EDTA 20.60 g 41.2 -
Distilled water 500.00 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.5
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Associated strains

All strains for this medium: 2
Tepidimonas sp. DSM 14591

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Sodium acetate 0.495 6.029
(NH4)2SO4 0.495 3.743
Na2S2O3 0.495 3.128
Sodium succinate 0.495 3.052
Na glutamate 0.495 2.588
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Cultivation metadata from 2 strains BacDive

pH 6 - 8 1 strain
pH 8 - 10 1 strain
phyla 2 2 strains
nutrition chemoheterotroph 1 strain
nutrition photoheterotroph 1 strain
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