Compound | Amount | Unit | |||
---|---|---|---|---|---|
|
Glucose | 10.0 | g | ||
|
Polypeptone
(Nihon Pharm. Co.) |
5.0 | g | ||
|
Yeast extract | 5.0 | g | ||
|
NaCl | 10.0 | g | ||
|
K2HPO4 | 1.0 | g | ||
|
MgSO4 x 7 H2O | 0.2 | g | ||
|
Agar | 15.0 | g | ||
|
Distilled water | 900.0 | ml | ||
1 After autoclaving, aseptically add 100 ml of a sterile 10% NaHCO3 solution to the medium. Check final pH to be about 9.0. |