J1213
1213 .
PYG MEDIUM (J), MODIFIED
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J1213 #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Trypticase peptone | 5.0 | g | 4.995 | - | |
| Peptone | 5.0 | g | 4.995 | - | |
| Yeast extract | 10.0 | g | 9.99 | - | |
| Beef extract | 5.0 | g | 4.995 | - | |
| Glucose | 5.0 | g | 4.995 | 27.725 | |
| KH2PO4 | 2.0 | g | 1.998 | 14.682 | |
| Tween 80 | 1.0 | ml | - | - | |
| Resazurin | 1.0 | mg | 1.0e-3 | 0.004 | |
| Salt solution | 40.0 | ml | - | - | |
| Distilled water | 890.0 | ml | - | - | |
| 1 Mix components thoroughly, bring to a boil and cool down under a stream of O2-free CO2. While gassing, add the following solutions and adjust pH to 7.2: | |||||
|
Rumen fluid
, clarified
(see Medium No. 266) |
50.0 | ml | - | - | |
|
L-Cysteine HCl x H2O
(5%) |
10.0 | ml | - | - | |
| Hemin solution | 10.0 | ml | - | - | |
| Vitamin K1 solution | 0.2 | ml | - | - | |
| 2 Distribute the medium into culture vessels (e.g., 10 ml in Balch type tubes) under N2, seal with butyl rubber stoppers and autoclave. | |||||
Equipment needed: Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Vitamin K1 | 0.1 | ml | - | - | |
|
Ethanol
(95 %) |
20.0 | ml | 950 | 20620.8 | |
| 1 Dissolve 0.1 ml of vitamin K1 in 20 ml 95% ethanol and filter sterilize. Store refrigerated in a brown bottle. | |||||
Solution occurs in 9
other media (first defined in Medium 104).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| CaCl2 x 2 H2O | 0.25 | g | 0.25 | 1.701 |
| MgSO4 x 7 H2O | 0.50 | g | 0.5 | 2.029 |
| K2HPO4 | 1.00 | g | 1 | 5.741 |
| KH2PO4 | 1.00 | g | 1 | 7.348 |
| NaHCO3 | 10.00 | g | 10 | 119.038 |
| NaCl | 2.00 | g | 2 | 34.223 |
| Distilled water | 1000.00 | ml | - | - |
Solution occurs in 5
other media (first defined in Medium J469).
| 1 Dissolve 50 mg hemin first in 1 ml of 1 N NaOH. Then adjust volume to 100 ml. |
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Medium type: | Complex medium |
| Final pH: | 7.2 |
| Equipment needed: | Autoclave, Filter |
| Edit in Medium builder | |