1035 .
VXG GELLAN
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Equipment needed:
Final pH:
5.5
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
MES | 1.95 | g | ||
|
|
MgSO4 x 7 H2O
(20 mM) |
10.00 | ml | ||
|
|
CaCl2 x 2 H2O
(30 mM) |
10.00 | ml | ||
|
|
(NH4)2HPO4
(20 mM) |
10.00 | ml | ||
|
|
MgCl2 x 6 H2O
(1 M) |
10.00 | ml | ||
|
|
Selenite-tungstate solution | 1.00 | ml | ||
|
|
Trace element solution SL-10 | 1.00 | ml | ||
|
|
Xylan | 0.50 | g | ||
|
|
Gellan Gum | 8.00 | g | ||
| 1 Adjust pH to 5.5 | |||||
Selenite-tungstate solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
NaOH | 0.5 | g |
|
|
Na2SeO3 x 5 H2O | 3.0 | mg |
|
|
Na2WO4 x 2 H2O | 4.0 | mg |
|
|
Distilled water | 1000.0 | ml |
Trace element solution SL-10 #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
HCl
(25%, 7.7 M) |
10.0 | ml | ||
|
|
FeCl2 x 4 H2O | 1.5 | g | ||
|
|
ZnCl2 | 70.0 | mg | ||
|
|
MnCl2 x 4 H2O | 100.0 | mg | ||
|
|
H3BO3 | 6.0 | mg | ||
|
|
CoCl2 x 6 H2O | 190.0 | mg | ||
|
|
CuCl2 x 2 H2O | 2.0 | mg | ||
|
|
NiCl2 x 6 H2O | 24.0 | mg | ||
|
|
Na2MoO4 x 2 H2O | 36.0 | mg | ||
|
|
Distilled water | 990.0 | ml | ||
| 1 Dissolve FeCl2 x 4H2O first in HCl. Then dilute in distilled water and add the remaining salts. | |||||