# J1035: VXG GELLAN


## Main sol. J1035

1.95	g	MES	
10	ml	MgSO4 x 7 H2O (20 mM)	
10	ml	CaCl2 x 2 H2O (30 mM)	
10	ml	(NH4)2HPO4 (20 mM)	
10	ml	MgCl2 x 6 H2O (1 M)	
1	ml	**Selenite-tungstate solution**	
1	ml	**Trace element solution SL-10**	
0.5	g	Xylan	
8	g	Gellan Gum	

1. Adjust pH to 5.5


## Selenite-tungstate solution

0.5	g	NaOH	
3	mg	Na2SeO3 x 5 H2O	
4	mg	Na2WO4 x 2 H2O	
1000	ml	Distilled water	


## Trace element solution SL-10

10	ml	HCl (25%, 7.7 M)	
1.5	g	FeCl2 x 4 H2O	
70	mg	ZnCl2	
100	mg	MnCl2 x 4 H2O	
6	mg	H3BO3	
190	mg	CoCl2 x 6 H2O	
2	mg	CuCl2 x 2 H2O	
24	mg	NiCl2 x 6 H2O	
36	mg	Na2MoO4 x 2 H2O	
990	ml	Distilled water	

1. Dissolve FeCl2 x 4H2O first in HCl. Then dilute in distilled water and add the remaining salts.