CCAP 8 . ASW 300 + barley

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Equipment needed:

Final pH: n.d.

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ml
Compound Amount Unit
NaCl 243.2 g
KCl 7.6 g
MgCl2 x 6 H2O 54.4 g
MgSO4 x 7 H2O 59.4 g
CaCl2 x 2 H2O 1.3 g
1 Add 20 ml of the autoclaved artificial seawater with 300 psu and 4 autoclaved barley grains to a T­25 tissue culture flask (Corning 25100).