# C8: ASW 300 + barley


## Mail sol. C8

243.2	g	NaCl	
7.6	g	KCl	
54.4	g	MgCl2 x 6 H2O	
59.4	g	MgSO4 x 7 H2O	
1.3	g	CaCl2 x 2 H2O	

1. Add 20 ml of the autoclaved artificial seawater with 300 psu and 4 autoclaved 
barley grains to a T­25 tissue culture flask (Corning 25100).