993b
993b .
MODIFIED ISP5 MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Medium for Yaniella halotolerans DSM 15476
This strain uses the standard medium without modifications.
Growth conditions: 28 °C
Main sol. 993b #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Medium 993 | 1000.0 | ml | - | - | |
| Yeast extract | 0.5 | % | 5 | - | |
| KCl | 10.0 | % | 100 | 1341.38 | |
| 1 Prepare medium 993 supplemented with yeast extract (0.5 %) and KCl (10%). pH of the medium should be 7.2. | |||||
Solution occurs in 3
other media (first defined in Medium 993).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
L-Asparagine
(anhydrous basis) |
1 | g | 1 | 7.569 | |
| Glycerol | 10 | g | 10 | 108.589 | |
|
K2HPO4
(anhydrous basis) |
1 | g | 1 | 5.741 | |
| Distilled water | 1000 | ml | - | - | |
| Trace element solution | 1 | ml | - | - | |
| Agar | 20 | g | 20 | 59.465 | |
| 1 The pH of trace salts solution is about 7.0-7.4. Do not adjust if it is within this range. Liquefy agar by steaming at 100°C for 15-20 minutes. | |||||
| 2 Sterilize in flasks for pouring into Petri dishes. Seven standard 90 mm dishes containing 25 ml per dish. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| FeSO4 x 7 H2O | 0.1 | g | 1 | 3.597 |
| MnCl2 x 4 H2O | 0.1 | g | 1 | 5.053 |
| ZnSO4 x 7 H2O | 0.1 | g | 1 | 3.478 |
| Distilled water | 100.0 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.2 |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 3 |
Yaniella halotolerans DSM 15476
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| KCl | 100 | 1341.38 |
| Agar | 20 | 59.465 |
| Glycerol | 10 | 108.589 |
| Yeast extract | 5 | - |
| L-Asparagine | 1 | 7.569 |
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