DSMZ 993a . NESTERENKONIA MODIFIED MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

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Medium for Nesterenkonia halotolerans DSM 15474

This strain uses the standard medium without modifications.


Growth conditions: 28 °C
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Medium 993 1000.0 ml - -
Yeast extract 0.5 % 5 -
MgCl2 x 6 H2O 10.0 % 100 491.877
1 Use medium 993 supplemented with Yeast extract (0.5%) and MgCl2 x 6 H20 (10%).
Solution occurs in 3 other media (first defined in Medium 993).
Compound Amount Unit Conc. [g/L] Conc. [mM]
L-Asparagine
(anhydrous basis)
1 g 1 7.569
Glycerol 10 g 10 108.589
K2HPO4
(anhydrous basis)
1 g 1 5.741
Distilled water 1000 ml - -
Trace element solution 1 ml - -
Agar 20 g 20 59.465
1 The pH of trace salts solution is about 7.0-7.4. Do not adjust if it is within this range. Liquefy agar by steaming at 100°C for 15-20 minutes.
2 Sterilize in flasks for pouring into Petri dishes. Seven standard 90 mm dishes containing 25 ml per dish.
Solution occurs in 8 other media (first defined in Medium 84).
Compound Amount Unit Conc. [g/L] Conc. [mM]
FeSO4 x 7 H2O 0.1 g 1 3.597
MnCl2 x 4 H2O 0.1 g 1 5.053
ZnSO4 x 7 H2O 0.1 g 1 3.478
Distilled water 100.0 ml - -

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.2
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 1
Nesterenkonia halotolerans DSM 15474

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
MgCl2 x 6 H2O 100 491.877
Agar 20 59.465
Glycerol 10 108.589
Yeast extract 5 -
L-Asparagine 1 7.569
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Cultivation metadata from 1 strains BacDive

pH 6 - 8 1 strain
pH 8 - 10 1 strain
phyla 1 1 strain
temperature mesophilic 1 strain