Name: MARINITOGA LITORALIS MEDIUM
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Vol.

Main sol. 959a #

Equipment needed: Autoclave Hungate tubes Gassing station

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
NaCl	26.00	g	26	444.901
MgCl₂ x 6 H₂O	0.50	g	0.5	2.459
CaCl₂ x 2 H₂O	0.10	g	0.1	0.68
NH₄Cl	1.00	g	1	18.695
KCl	0.20	g	0.2	2.683
PIPES (SIGMA)	3.40	g	3.4	11.245
K₂HPO₄	0.35	g	0.35	2.009
KH₂PO₄	0.35	g	0.35	2.572
Na-acetate x 3 H₂O	2.72	g	2.72	-
Yeast extract (OXOID)	2.00	g	2	-
Trypticase peptone (BD BBL)	2.00	g	2	-
Sodium resazurin (0.1% w/v)	0.50	ml	5.0e-4	-
Maltose	3.50	g	3.5	10.225
Na₂S x 9 H₂O	0.50	g	0.5	2.082
Distilled water	1000.00	ml	-	-
1 Dissolve ingredients except maltose and sulfide, adjust pH to 5.8 - 6.0 and sparge medium with 100% N₂ gas for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving add maltose and sulfide from sterile anoxic stock solutions prepared under 100% N₂ gas atmosphere. The pH of the complete medium should be 6.0.

Last modified:	11.04.22
Source:	DSMZ
Taxonomic range:	Bacteria
Medium type:	Complex medium
Final pH:	6.0
Equipment needed:	Autoclave, Gassing station, Hungate tubes

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Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

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959a
959a . MARINITOGA LITORALIS MEDIUM