927 .
HALORHABDUS UTAHENSIS MEDIUM
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Equipment needed:
Final pH:
7.6
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
NaCl | 270.00 | g | ||
|
|
NaBr | 0.10 | g | ||
|
|
MgSO4 x 7 H2O | 20.00 | g | ||
|
|
KCl | 5.00 | g | ||
|
|
NH4Cl | 2.00 | g | ||
|
|
Tris-HCl | 12.00 | g | ||
|
|
Yeast extract
(Difco) |
1.00 | g | ||
|
|
Glucose | 2.00 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
|
|
KH2PO4 solution 5%(w/v) | 2.50 | ml | ||
|
|
CaCl2 solution 10%(w/v) | 0.50 | ml | ||
|
|
FeCl2/MnCl2 solution | 0.25 | ml | ||
| 1 Prepare the medium, and adjust the pH to 7.6 with 5M NaOH. | |||||
| 2 Autoclave and when the medium has cooled add 2.5 ml of the phosphate solution, 0.5 ml of the calcium chloride solution, and 0.25 ml of the Fe/Mn solution. This strain will not grow in normal media for halobacteria, such as DSM medium 372! | |||||
Trace element solution SL-7 #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
HCl
(25%) |
6.5 | ml |
|
|
FeCl2 x 7 H2O | 1.5 | g |
|
|
ZnCl2 | 70.0 | mg |
|
|
MnCl2 x 4 H2O | 100.0 | mg |
|
|
H3BO3 | 62.0 | mg |
|
|
CoCl2 x 6 H2O | 190.0 | mg |
|
|
CuCl2 x 2 H2O | 17.0 | mg |
|
|
NiCl2 x 6 H2O | 24.0 | mg |
|
|
Na2MoO4 x 2 H2O | 36.0 | mg |
|
|
Distilled water | 1000.0 | ml |
KH2PO4 solution 5%(w/v) #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
KH2PO4 | 50 | g | ||
|
|
Distilled water | 1000 | ml | ||
| 1 Prepare and sterilise separately | |||||
CaCl2 solution 10%(w/v) #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
CaCl2 x 6 H2O | 100 | g | ||
|
|
Distilled water | 1000 | ml | ||
| 1 Prepare and sterilise separately | |||||
FeCl2/MnCl2 solution #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
FeCl2 x 4 H2O | 20 | g | ||
|
|
MnCl2 x 4 H2O | 20 | g | ||
|
|
Distilled water | 1000 | ml | ||
| 1 Prepare and sterilise separately | |||||