# 927: HALORHABDUS UTAHENSIS MEDIUM


## Main sol. 927

270	g	NaCl	
0.1	g	NaBr	
20	g	MgSO4 x 7 H2O	
5	g	KCl	
2	g	NH4Cl	
12	g	Tris-HCl	
1	g	Yeast extract (Difco)	
2	g	Glucose	
1000	ml	Distilled water	
2.5	ml	**KH2PO4 solution 5%(w/v)**	
0.5	ml	**CaCl2 solution 10%(w/v)**	
0.25	ml	**FeCl2/MnCl2 solution**	

1. Prepare the medium, and adjust the pH to 7.6 with 5M NaOH.
2. Autoclave and when the medium has cooled add 2.5 ml of the phosphate solution, 0.5 ml of the calcium chloride solution, and 0.25 ml of the Fe/Mn solution. This strain will not grow in normal media for halobacteria, such as DSM medium 372!


## Trace element solution SL-7

6.5	ml	HCl (25%)	
1.5	g	FeCl2 x 7 H2O	
70	mg	ZnCl2	
100	mg	MnCl2 x 4 H2O	
62	mg	H3BO3	
190	mg	CoCl2 x 6 H2O	
17	mg	CuCl2 x 2 H2O	
24	mg	NiCl2 x 6 H2O	
36	mg	Na2MoO4 x 2 H2O	
1000	ml	Distilled water	


## KH2PO4 solution 5%(w/v)

50	g	KH2PO4	
1000	ml	Distilled water	

1. Prepare and sterilise separately


## CaCl2 solution 10%(w/v)

100	g	CaCl2 x 6 H2O	
1000	ml	Distilled water	

1. Prepare and sterilise separately


## FeCl2/MnCl2 solution

20	g	FeCl2 x 4 H2O	
20	g	MnCl2 x 4 H2O	
1000	ml	Distilled water	

1. Prepare and sterilise separately