925 .
ALKALIPHILIC SULPHUR RESPIRING STRAINS MEDIUM
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* Medium for Thioalkalispira microaerophila DSM 14786
Thioalkalispira microaerophila, ALEN 1 = DSM 14786. The strain is grown under nitrogen with 1% oxygen in the gas phase. The strain grows slowly with a potential lag phase of several days. Use 30 mM thiosulphate and up to a maximum of 5 mM NH4Cl
Growth conditions: 28 °C
Equipment needed:
Final gas composition:
1 % O2
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Final pH:
10.0
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
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Na2CO3 | 20 | g | ||
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NaHCO3 | 10 | g | ||
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NaCl | 5 | g | ||
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K2HPO4 | 1 | g | ||
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Trace element solution | 2 | ml/l | ||
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Distilled water | 1000 | ml | ||
| 1 Sterilize at 110°C 20 min in a closed vessel (i.e. a serum tube or bottle). pH after sterilization will about 10. | |||||
| 2 After sterilization add: | |||||
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MgCl2 x 6 H2O
(200.0 g/l) |
1 | ml/l | ||
| 3 (a white colloid will form which will rapidly dissolve after mixing) | |||||
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Sodium thiosulphate | 30 | mM | ||
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NH4Cl | 5 | mM | ||
Trace element solution #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
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EDTA | 5 | mg | ||
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FeSO4 x 7 H2O | 2 | mg | ||
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ZnSO4 x 7 H2O | 100 | mg | ||
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MnCl2 x 4 H2O | 30 | mg | ||
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CoCl2 x 6 H2O | 200 | mg | ||
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NiCl2 x 6 H2O | 20 | mg | ||
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Na2MoO4 x 2 H2O | 30 | mg | ||
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CuCl2 x 2 H2O | 10 | mg | ||
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H3BO3 | 300 | mg | ||
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Distilled water | 1000 | ml | ||
| 1 Final pH should be 3, add HCl if needed. Sterilization - 120°C 20 min. | |||||