925 .
ALKALIPHILIC SULPHUR RESPIRING STRAINS MEDIUM
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* Medium for Thioalkalivibrio nitratis DSM 13741
Thioalkalivibrio versutus AL 2 = DSM 13738, Tv.nitratus ALJ 12 = DSM 13741, and Tv.denitrificans ALJD = DSM 13742 Use 40 mM thiosulphate, 5 mM KNO3, reduce the amount of MgCl2 to 0.5 mM
Growth conditions: 30 °C
Equipment needed:
Final pH:
10.0
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Na2CO3 | 20.0 | g | ||
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NaHCO3 | 10.0 | g | ||
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NaCl | 5.0 | g | ||
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K2HPO4 | 1.0 | g | ||
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Trace element solution | 2.0 | ml/l | ||
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Distilled water | 1000.0 | ml | ||
| 1 Sterilize at 110°C 20 min in a closed vessel (i.e. a serum tube or bottle). pH after sterilization will about 10. | |||||
| 2 After sterilization add: | |||||
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MgCl2 x 6 H2O
(200.0 g/l) |
0.5 | mM | ||
| 3 (a white colloid will form which will rapidly dissolve after mixing) | |||||
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Sodium thiosulphate | 40.0 | mM | ||
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KNO3 | 5.0 | mM | ||
Trace element solution #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
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EDTA | 5 | mg | ||
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FeSO4 x 7 H2O | 2 | mg | ||
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ZnSO4 x 7 H2O | 100 | mg | ||
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MnCl2 x 4 H2O | 30 | mg | ||
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CoCl2 x 6 H2O | 200 | mg | ||
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NiCl2 x 6 H2O | 20 | mg | ||
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Na2MoO4 x 2 H2O | 30 | mg | ||
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CuCl2 x 2 H2O | 10 | mg | ||
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H3BO3 | 300 | mg | ||
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Distilled water | 1000 | ml | ||
| 1 Final pH should be 3, add HCl if needed. Sterilization - 120°C 20 min. | |||||