887
887 .
OS MEDIUM
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Medium for Thermocrinis albus DSM 12173
* Modification: incubate under gas atmosphere of 3% H2, 3% O2 in N2 (3 bar excess pressure)
The modifications for this strain were extracted automatically and have not been curated by experts, yet.
Growth conditions: 2-3 days
80 °C
Main sol. 887 #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 256.00 | mg | 0.256 | 4.381 | |
| KH2PO4 | 1.70 | mg | 0.002 | 0.012 | |
| CaCl2 x 2 H2O | 0.80 | mg | 8.0e-4 | 0.005 | |
| H3BO3 | 10.30 | mg | 0.01 | 0.167 | |
| Na2SO4 | 23.00 | mg | 0.023 | 0.162 | |
| NaNO3 | 0.30 | mg | 3.0e-4 | 0.004 | |
| KCl | 15.00 | mg | 0.015 | 0.201 | |
| FeCl3 x 6 H2O | 0.10 | mg | 1.0e-4 | 3.7e-4 | |
| MnSO4 x 4 H2O | 0.06 | mg | 6.0e-5 | 2.7e-4 | |
| Modified Wolin's mineral solution II | 10.00 | ml | - | - | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Sparge medium with 100% N2 gas mixture for 30 min to make anoxic. | |||||
| 2 Adjust pH to 7.0 with H2SO4. | |||||
| 3 Fill 10 ml medium into 100 ml Serum bottles under a stream of H2/CO2 gas and seal with a rubber stopper. | |||||
| 4 Autoclave at 121 °C for 20 min. | |||||
| 5 After autoclaving add the following (e.g., from sterile stock solutions) | |||||
|
Na2S2O3 x 5 H2O
(10% in H2O) |
1.00 | g/l | 1 | 4.029 | |
|
NaHCO3
(5 % in H2O) |
1.00 | g/l | 1 | 11.904 | |
| 6 Adjust the gas atmosphere strain specifically. | |||||
Solution occurs in 24
other media (first defined in Medium 700).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Nitrilotriacetic acid | 1.500 | g | 1.5 | 7.848 | |
| MgSO4 x 7 H2O | 3.000 | g | 3 | 12.172 | |
| MnSO4 x H2O | 0.500 | g | 0.5 | 2.958 | |
| NaCl | 1.000 | g | 1 | 17.112 | |
| FeSO4 x 7 H2O | 0.100 | g | 0.1 | 0.36 | |
| CoSO4 x 7 H2O | 0.180 | g | 0.18 | 1.161 | |
| CaCl2 x 2 H2O | 0.100 | g | 0.1 | 0.68 | |
| ZnSO4 x 7 H2O | 0.180 | g | 0.18 | 0.626 | |
| CuSO4 x 5 H2O | 0.010 | g | 0.01 | 0.04 | |
| AlK(SO4)2 x 12 H2O | 0.020 | g | 0.02 | 0.042 | |
| H3BO3 | 0.010 | g | 0.01 | 0.162 | |
| Na2MoO4 x 2 H2O | 0.010 | g | 0.01 | 0.041 | |
| NiCl2 x 6 H2O | 0.025 | g | 0.025 | 0.193 | |
| Na2SeO3 x 5 H2O | 0.300 | mg | 3.0e-4 | 0.001 | |
| Distilled water | 1000.000 | ml | - | - | |
| 1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH. | |||||
Metadata
| Last modified: | 21.02.24 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.0 |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |
Gas composition
- 94 % N2
- 3 % H2
- 3 % O2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaHCO3 | 1 | 11.904 |
| Na2S2O3 x 5 H2O | 1 | 4.029 |
| MgSO4 x 7 H2O | 0.03 | 0.122 |
| Na2SO4 | 0.023 | 0.162 |
| KCl | 0.015 | 0.201 |
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