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DSMZ

DSMZ 87 . CHLOROFLEXUS MEDIUM (modified)

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
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Medium for Chloroflexus aurantiacus DSM 635

This strain uses the standard medium without modifications.

Growth conditions: 3-7 days anaerobic 49 °C

Main sol. 87 #

ml
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Yeast extract 1.00 g 0.947 -
Glycyl-glycine 1.00 g 0.947 -
Na2HPO4 x 2 H2O 0.10 g 0.095 0.667
MgSO4 x 7 H2O 0.10 g 0.095 0.384
KNO3 0.10 g 0.095 0.937
NaNO3 0.50 g 0.473 5.571
NaCl 0.10 g 0.095 1.62
CaCl2 x 2 H2O 0.05 g 0.047 0.322
Fe(III) citrate
(0.1 g in 100 ml H2O) 		5.00 ml - -
Trace element solution SL-6 1.00 ml - -
Distilled water 1050.00 ml - -
1 Adjust pH to 8.2.
2 Boil the medium under a stream of nitrogen gas for a few minutes and distribute 90 ml medium into 100 ml screw-capped bottles. Bubble each bottle with nitrogen gas and close immediately with a rubber septum and screw tight. Autoclave at 121°C for 15 min. After autoclaving inject 1.0 ml of neutralized sulfide solution (0.03% end concentration) to each bottle. This medium can be stored for several months. Incubate the culture at 50°C at a light intensity of 300 - 500 lux. For heavy cell suspension supplement periodically with sterile yeast extract solution (0.1% end concentration, for more details see Ref. 3365, 3366).

Neutralized sulfide solution 3% (w/v) #

100
Solution occurs in 18 other media (first defined in Medium 28).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2S x 9 H2O 3 g 30 124.905
Distilled water 100 ml - -
1 The sulfide solution is prepared in a 250 ml screw-capped bottle with a butyl rubber septum and a magnetic stirrer. The solution is bubbled with nitrogen gas, closed and autoclaved for 15 min. at 121°C. After cooling to room temperature the pH is adjusted to about 7.0 by adding of sterile 2 M H2SO4 drop-wise with a syringe without opening the bottle.

Trace element solution SL-6 #

1000
Solution occurs in 55 other media (first defined in Medium 27).
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
MnCl2 x 4 H2O 0.03 g 0.03 0.152
H3BO3 0.30 g 0.3 4.851
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 x 2 H2O 0.01 g 0.01 0.059
NiCl2 x 6 H2O 0.02 g 0.02 0.154
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
Distilled water 1000.00 ml - -

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 8.2
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 4
Chloroflexus aurantiacus DSM 635

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Yeast extract 0.947 -
Glycyl-glycine 0.947 -
NaNO3 0.473 5.571
NaCl 0.095 1.62
KNO3 0.095 0.937
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Cultivation metadata from 4 strains BacDive

phyla 1 4 strains
oxygen anaerobe 4 strains
temperature thermophilic 4 strains