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DSMZ

DSMZ 87a . CHLOROFLEXUS AGGREGANS MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 87a #

ml
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Yeast extract 1.00 g 0.952 -
Glycyl-glycine 1.00 g 0.952 -
Na2HPO4 x 2 H2O 0.10 g 0.095 0.671
MgSO4 x 7 H2O 0.10 g 0.095 0.386
KNO3 0.10 g 0.095 0.942
NaNO3 0.50 g 0.476 5.603
NaCl 0.10 g 0.095 1.63
CaCl2 x 2 H2O 0.05 g 0.048 0.324
Fe(III) citrate
(0.1 g in 100 ml H2O) 		5.00 ml - -
Trace element solution SL-6 1.00 ml - -
Vitamin solution CA 1.00 ml - -
Distilled water 1050.00 ml - -
1 Adjust pH to 8.2.
2 Boil the medium under a stream of nitrogen gas for a few minutes and distribute 90 ml medium into 100 ml screw-capped bottles. Bubble each bottle with nitrogen gas and close immediately with a rubber septum and screw tight. Autoclave at 121°C for 15 min. After autoclaving inject 1.0 ml of neutralized sulfide solution (0.03% end concentration) to each bottle. This medium can be stored for several months. Incubate the culture at 50°C at a light intensity of 300 - 500 lux. For heavy cell suspension supplement periodically with sterile yeast extract solution (0.1% end concentration, for more details see Ref. 3365, 3366).

Vitamin solution CA #

100
Solution occurs in 1 other media (first defined in Medium ).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Distilled water 100 ml - -
Nicotinic acid 100 mg 1 8.123
Thiamine-HCl x 2 H2O 100 mg 1 2.679
Biotin 5 mg 0.05 0.205
p-Aminobenzoic acid 50 mg 0.5 3.646
Vitamin B12 1 mg 0.01 0.007
Calcium pantothenate 50 mg 0.5 1.049
Pyridoxine hydrochloride 50 mg 0.5 2.431
Folic acid 50 mg 0.5 1.133
Na3-EDTA 200 mg 2 5.584
1 Adjust pH to 7.5. The solution is filter-sterilized.

Trace element solution SL-6 #

1000
Solution occurs in 55 other media (first defined in Medium 27).
Compound Amount Unit Conc. [g/L] Conc. [mM]
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
MnCl2 x 4 H2O 0.03 g 0.03 0.152
H3BO3 0.30 g 0.3 4.851
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 x 2 H2O 0.01 g 0.01 0.059
NiCl2 x 6 H2O 0.02 g 0.02 0.154
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
Distilled water 1000.00 ml - -

Neutralized sulfide solution 3%(w/v) #

100
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Distilled water 100 ml - -
Na2S x 9 H2O 3 g 30 124.905
1 The sulfide solution is prepared in a 250 ml screw-capped bottle with a butyl rubber septum and a magnetic stirrer. The solution is bubbled with nitrogen gas, closed and autoclaved for 15 min. at 121°C. After cooling to room temperature the pH is adjusted to about 7.0 by adding of sterile 2 M H2SO4 drop-wise with a syringe without opening the bottle.
2 Appearance of a yellow colour indicates the drop of pH to about 8. The solution should be stirred continuously to avoid precipitation of elemental sulfur. The final solution should be clear and is yellow in colour.

Metadata

Last modified: 24.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 8.2
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 3
Strains with modifications: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Glycyl-glycine 0.952 -
Yeast extract 0.952 -
NaNO3 0.476 5.603
NaCl 0.095 1.63
KNO3 0.095 0.942
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Cultivation metadata from 3 strains BacDive

pH 6 - 8 1 strain
pH 8 - 10 1 strain
phyla 1 3 strains
nutrition chemoheterotroph 2 strains
nutrition photoheterotroph 2 strains
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