804
804 .
HAEMOPHILUS MEDIUM
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
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Medium for Haemophilus influenzae DSM 23393
This strain uses the standard medium without modifications.
Growth conditions: 1-2 days
microaerobic
37 °C
pH 7.00
Main sol. 804 #
ml
Equipment needed: Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Mueller-Hinton broth | 21 | g | 21 | - | |
| Yeast extract | 5 | g | 5 | - | |
| Distilled water | 1000 | ml | - | - | |
| 1 For Haemophilus spp., add 15 mg NAD and 15 mg hemine per L (X + V factors). | |||||
| 2 NAD stock solution: dissolve 1 mg NAD per ml dist. water, filter sterilize. | |||||
| 3 Hemine stock solution: dissolve 50 mg hemine in 1 ml 1 M NaOH, make up to 25 ml with dist. water, filter sterilize. | |||||
Metadata
| Last modified: | 09.01.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | n.d. |
| Equipment needed: | Filter |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 39 |
| Strains with modifications: | 2 |
Haemophilus influenzae DSM 23393
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Mueller-Hinton broth | 21 | - |
| Yeast extract | 5 | - |
| Distilled water | - | - |
Cultivation metadata from 39 strains
| phyla | 2 | 40 strains |
| oxygen | microaerophile | 24 strains |
| oxygen | facultative anaerobe | 10 strains |
| oxygen | aerobe | 2 strains |
| oxygen | obligate aerobe | 1 strain |
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