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Main sol. 804

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Internal Solution-ID: S1635

Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Mueller-Hinton broth 21 g 21 -
Yeast extract 5 g 5 -
Distilled water 1000 ml - -
1 For Haemophilus spp., add 15 mg NAD and 15 mg hemine per L (X + V factors).
2 NAD stock solution: dissolve 1 mg NAD per ml dist. water, filter sterilize.
3 Hemine stock solution: dissolve 50 mg hemine in 1 ml 1 M NaOH, make up to 25 ml with dist. water, filter sterilize.
804 HAEMOPHILUS MEDIUM