779
779 .
CAPNOCYTOPHAGA II MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Medium for Capnocytophaga canimorsus DSM 19204
This strain uses the standard medium without modifications.
Growth conditions: 2-3 days
microaerobic
37 °C
pH 7.00
Main sol. 779 #
ml
Equipment needed: Autoclave
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Lab-Lemco beef extract
(Oxoid) |
2.4 | g | 2.4 | - | |
|
Proteose peptone no. 3
(Difco) |
10.0 | g | 10 | - | |
|
Yeast extract
(Difco, Bacto) |
5.0 | g | 5 | - | |
| Na2HPO4 | 4.0 | g | 4 | 28.177 | |
| Glucose | 1.5 | g | 1.5 | 8.326 | |
|
Starch
(soluble) |
0.5 | g | 0.5 | - | |
| L-Cysteine HCl x H2O | 0.5 | g | 0.5 | 2.847 | |
| Agar , if necessary | 15.0 | g | 15 | 44.599 | |
| Horse blood | 50.0 | ml | - | - | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Adjust pH to 7.6 - 7.8. | |||||
| 2 Add the horse blood to the heat sterilized medium after being cooled to 50°C. Incubate under air plus 5% CO2 or anaerobically under 95% N2 plus 5% CO2. | |||||
Metadata
| Last modified: | 19.04.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.6 - 7.8 |
| Equipment needed: | Autoclave, Gassing station |
| Edit in Medium builder | |
Gas composition
- 95 % N2
- 5 % CO2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Associated strains
| All strains for this medium: | 3 |
Capnocytophaga canimorsus DSM 19204
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Agar | 15 | 44.599 |
| Proteose peptone no. 3 | 10 | - |
| Yeast extract | 5 | - |
| Na2HPO4 | 4 | 28.177 |
| Lab-Lemco beef extract | 2.4 | - |
| Show more | ||
