693
693 .
COLUMBIA BLOOD MEDIUM
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Medium for Eubacterium nodatum DSM 3993
This strain uses the standard medium without modifications.
Growth conditions: 2-3 days
anaerobic
37 °C
pH 7.00
Main sol. 693 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Columbia agar base | 1000 | ml | - | - | |
| Defibrinated sheep blood | 5 | % | 50 | - | |
| 1 Columbia agar base supplemented with 5% defibrinated sheep blood. Rehydrate pellets of freeze-dried ampoules in liquid medium 545, 215 or 1 (see below). | |||||
| 2 ATTENTION: organisms, for which only M 693 is mentioned in the catalogue entry, do not grow in one of the media listed below! Those are meant to rehydrate the freeze-dried pellets, not for cultivation. | |||||
Solution occurs in 4
other media (first defined in Medium 54a).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Casein peptone | 17.0 | g | 17 | - | |
| Soy peptone | 3.0 | g | 3 | - | |
| D(+)-Glucose | 2.5 | g | 2.5 | 13.877 | |
| NaCl | 5.0 | g | 5 | 85.558 | |
| K2HPO4 | 2.5 | g | 2.5 | 14.353 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Adjust pH to 7.3. | |||||
| 2 The same as Caso Bouillon (Merck or Oxoid, Germany). | |||||
Solution occurs in 2
other media (first defined in Medium 429).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
|
Brain heart infusion
(Difco) |
37 | g | 37 | - |
| Distilled water | 1000 | ml | - | - |
Solution occurs in 2
other media (first defined in Medium 429).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Peptone | 5 | g | 5 | - | |
| Meat extract | 3 | g | 3 | - | |
| Distilled water | 1000 | ml | - | - | |
| 1 Adjust pH to 7.0. | |||||
Metadata
| Last modified: | 24.11.21 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | n.d. |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 2436 |
| Strains with modifications: | 87 |
Eubacterium nodatum DSM 3993
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Defibrinated sheep blood | 50 | - |
| Columbia agar base | - | - |
Cultivation metadata from 2436 strains
| halophily | halotolerant | 1 strain |
| halophily | non-halophilic | 1 strain |
| pH | 6 - 8 | 65 strains |
| pH | 8 - 10 | 34 strains |
| pH | 4 - 6 | 32 strains |
| Show more | ||