640
640 .
CALDICELLULOSIRUPTOR MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Medium for Clostridium sp. DSM 25153
This strain uses the standard medium without modifications.
Growth conditions: anaerobic
60 °C
Main sol. 640 #
ml
Equipment needed: Hungate tubes
Filter
Autoclave
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NH4Cl | 0.90 | g | 0.9 | 16.826 | |
| NaCl | 0.90 | g | 0.9 | 15.4 | |
| MgCl2 x 6 H2O | 0.40 | g | 0.4 | 1.968 | |
| KH2PO4 | 0.75 | g | 0.75 | 5.511 | |
| K2HPO4 | 1.50 | g | 1.5 | 8.612 | |
|
Trypticase peptone
(BD BBL) |
2.00 | g | 2 | - | |
|
Yeast extract
(OXOID) |
1.00 | g | 1 | - | |
| Trace element solution SL-10 | 1.00 | ml | - | - | |
|
FeCl3 x 6 H2O
(0.1% w/v in 0.2 N HCl) |
2.50 | ml | 0.003 | 0.009 | |
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.0e-4 | - | |
| L-Cysteine HCl x H2O | 0.75 | g | 0.75 | 4.27 | |
| Cellobiose | 1.00 | g | 1 | 2.921 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients except cysteine and cellobiose. Sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic, then add cysteine and adjust pH to 7.2. Distribute medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add cellobiose after autoclaving from an anoxic stock solution prepared under 100% N2 gas atmosphere and sterilized by filtration. Adjust pH of the complete medium to 7.2, if necessary. | |||||
Solution occurs in 266
other media (first defined in Medium 320).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
HCl
(25%) |
10.0 | ml | 2.5 | 68.568 | |
| FeCl2 x 4 H2O | 1.5 | g | 1.5 | 7.545 | |
| ZnCl2 | 70.0 | mg | 0.07 | 0.514 | |
| MnCl2 x 4 H2O | 100.0 | mg | 0.1 | 0.505 | |
| H3BO3 | 6.0 | mg | 0.006 | 0.097 | |
| CoCl2 x 6 H2O | 190.0 | mg | 0.19 | 1.463 | |
| CuCl2 x 2 H2O | 2.0 | mg | 0.002 | 0.012 | |
| NiCl2 x 6 H2O | 24.0 | mg | 0.024 | 0.185 | |
| Na2MoO4 x 2 H2O | 36.0 | mg | 0.036 | 0.149 | |
| Distilled water | 990.0 | ml | - | - | |
| 1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml. | |||||
Metadata
| Last modified: | 05.04.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.2 |
| Equipment needed: | Autoclave, Filter, Gassing station, Hungate tubes |
| Edit in Medium builder | |
Gas composition
- 100 % N2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Associated strains
| All strains for this medium: | 24 |
| Strains with modifications: | 16 |
Clostridium sp. DSM 25153
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Trypticase peptone | 2 | - |
| K2HPO4 | 1.5 | 8.612 |
| Cellobiose | 1 | 2.921 |
| Yeast extract | 1 | - |
| NH4Cl | 0.9 | 16.826 |
| Show more | ||
Cultivation metadata from 24 strains
| pH | 6 - 8 | 1 strain |
| phyla | 1 | 24 strains |
| oxygen | anaerobe | 18 strains |
| temperature | thermophilic | 23 strains |
| temperature | mesophilic | 1 strain |