Equipment needed: Hungate tubes
Filter
Autoclave
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NH4Cl | 0.90 | g | 0.9 | 16.826 | |
| NaCl | 0.90 | g | 0.9 | 15.4 | |
| MgCl2 x 6 H2O | 0.40 | g | 0.4 | 1.968 | |
| KH2PO4 | 0.75 | g | 0.75 | 5.511 | |
| K2HPO4 | 1.50 | g | 1.5 | 8.612 | |
|
Trypticase peptone
(BD BBL) |
2.00 | g | 2 | - | |
|
Yeast extract
(OXOID) |
1.00 | g | 1 | - | |
| Trace element solution SL-10 | 1.00 | ml | - | - | |
|
FeCl3 x 6 H2O
(0.1% w/v in 0.2 N HCl) |
2.50 | ml | 0.003 | 0.009 | |
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.0e-4 | - | |
| L-Cysteine HCl x H2O | 0.75 | g | 0.75 | 4.27 | |
| Cellobiose | 1.00 | g | 1 | 2.921 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients except cysteine and cellobiose. Sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic, then add cysteine and adjust pH to 7.2. Distribute medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add cellobiose after autoclaving from an anoxic stock solution prepared under 100% N2 gas atmosphere and sterilized by filtration. Adjust pH of the complete medium to 7.2, if necessary. | |||||
Media with this solution
| 640 | CALDICELLULOSIRUPTOR MEDIUM |